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  4. Rat oxidized low density lipoprotein,OxLDL ELISA Kit

Rat oxidized low density lipoprotein,OxLDL ELISA Kit

  • 中文名稱：
    大鼠氧化低密度脂蛋白(OxLDL)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E07932r
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3900/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠氧化低密度脂蛋白(OxLDL)酶聯(lián)免疫試劑盒（CSB-E07932r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的OxLDL含量。OxLDL即氧化低密度脂蛋白，是體內(nèi)低密度脂蛋白被氧化修飾形成。它在動(dòng)脈粥樣硬化等疾病發(fā)展中扮演重要角色。研究顯示，OxLDL可被巨噬細(xì)胞攝取形成泡沫細(xì)胞，還能引發(fā)炎癥反應(yīng)、誘導(dǎo)細(xì)胞凋亡等，是心血管疾病研究的重要靶點(diǎn)。試劑盒檢測范圍為6.25 pg/mL-400 pg/mL，該產(chǎn)品適用于心血管疾病動(dòng)物模型研究、藥物干預(yù)效果評(píng)估、脂質(zhì)代謝相關(guān)機(jī)制探索及細(xì)胞氧化應(yīng)激實(shí)驗(yàn)等科研場景本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫：
    OxLDL
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍：
    6.25 pg/mL-400 pg/mL
  • 靈敏度：
    1.56 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Others
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat OxLDL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:100Average %92
    Range %87-96
    1:200Average %94
    Range %90-99
    1:400Average %100
    Range %96-106
    1:800Average %90
    Range %85-96
  • 回收率：
    The recovery of rat OxLDL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9690-100
    EDTA plasma (n=4)9189-94
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    4002.668 2.783 2.726 2.541
    2002.296 2.284 2.290 2.105
    1001.704 1.735 1.720 1.535
    501.076 1.083 1.080 0.895
    250.607 0.624 0.616 0.431
    12.50.408 0.417 0.413 0.228
    6.250.291 0.298 0.295 0.110
    00.184 0.186 0.185 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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