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  4. Mouse oxidized low density lipoprotein,OxLDL ELISA Kit

Mouse oxidized low density lipoprotein,OxLDL ELISA Kit

  • 中文名稱：
    小鼠氧化低密度脂蛋白(OxLDL)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E07933m
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠氧化低密度脂蛋白(OxLDL)酶聯(lián)免疫試劑盒（CSB-E07933m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的OxLDL含量。OxLDL即氧化低密度脂蛋白，是動脈粥樣硬化發(fā)生發(fā)展的關(guān)鍵因素。其背景在于體內(nèi)LDL被氧化修飾形成OxLDL。研究機(jī)制顯示，OxLDL可被巨噬細(xì)胞攝取，形成泡沫細(xì)胞，促進(jìn)脂質(zhì)條紋和斑塊形成，還能引發(fā)炎癥反應(yīng)，加速動脈粥樣硬化進(jìn)程。試劑盒檢測范圍為0.45 nmol/mL-30 nmol/mL，適用于體外實驗中對小鼠模型心血管疾病機(jī)制、脂質(zhì)代謝異?；蜓趸瘧?yīng)激相關(guān)研究的定量分析，尤其適用于動脈粥樣硬化動物模型中的動態(tài)監(jiān)測及藥物干預(yù)效果評估本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫：
    OxLDL
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.45 nmol/mL-30 nmol/mL
  • 靈敏度：
    0.11 nmol/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Others
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse OxLDL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:100Average %91
    Range %86-95
    1:200Average %97
    Range %92-103
    1:400Average %85
    Range %81-87
    1:800Average %89
    Range %83-96
  • 回收率：
    The recovery of mouse OxLDL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9287-96
    EDTA plasma (n=4)9589-99
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    nmol/mlOD1OD2AverageCorrected
    302.648 2.772 2.710 2.571
    152.139 2.130 2.135 1.996
    7.51.527 1.639 1.583 1.444
    3.750.885 0.866 0.876 0.737
    1.860.541 0.532 0.537 0.398
    0.940.378 0.405 0.392 0.253
    0.450.243 0.242 0.243 0.104
    00.136 0.142 0.139 　
  • 本試劑盒所含材料：
    • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-mouse OxLDL antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
    • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
    • One vial Biotin-labeled OxLDL antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
    • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
    • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
    • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
    • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
    • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
    • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
    • An instruction manual

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  • 本試劑盒不含材料：
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
    • An incubator can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution

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  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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相關(guān)問答

■ 常見問題解答
Q:

I would like to know which portion of OXLDL is recognized by your mouse oxLDL kit cat# CSB-E07933m.

A:
Thanks for your inquiry.
The standard of this kit is OXLDL prepared by the oxidation of cupric sulfate.
IIt can detect endogenous OXLDL.
At the same time, the OXLDL prepared by different methods can be detected as long as the corresponding recognition sites/regions are available.
Sorry we are not clear about the specific recognition area. If you want to detect other methods to prepare the OXLDL, we suggest you do a preliminary experiment.