Rabbit Interferon α,IFN-α ELISA Kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

兔子α干擾素(IFN-α)酶聯(lián)免疫試劑盒（CSB-E07329Rb）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IFNA1含量。IFNA1 即α1型干擾素，其具有抗病毒、免疫調節(jié)等多種作用。在研究機制方面，它可與細胞表面受體結合，激活下游信號通路，誘導多種抗病毒蛋白產(chǎn)生，發(fā)揮抑制病毒復制和調節(jié)機體免疫反應等功能，是抗病毒和免疫相關研究的重要靶點。試劑盒檢測范圍為6.25 pg/mL-400 pg/mL，適用于生命科學研究中病毒侵染模型構建、免疫調節(jié)機制解析以及疫苗/抗病毒藥物研發(fā)等場景，特別適用于兔科動物模型中天然免疫反應的動態(tài)監(jiān)測。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

IFN-alpha，interferon alpha
縮寫：

IFN-α
種屬：

Oryctolagus cuniculus (Rabbit)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

6.25 pg/mL-400 pg/mL
靈敏度：

1.56 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IFN-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	82-92
1:2	Average %	92
	Range %	88-98
1:4	Average %	95
	Range %	90-100
1:8	Average %	89
	Range %	86-94

回收率：

The recovery of rabbit IFN-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	84	80-88
EDTA plasma (n=4)	94	88-98

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.