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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Escherichia fergusonii LPS-assembly lipoprotein lptE (lptE) CSB-YP487547EOR
CSB-EP487547EOR
CSB-BP487547EOR
CSB-MP487547EOR
CSB-EP487547EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Fumarate reductase subunit D (frdD), partial CSB-YP487548EOR1
CSB-EP487548EOR1
CSB-BP487548EOR1
CSB-MP487548EOR1
CSB-EP487548EOR1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii HTH-type transcriptional repressor NsrR (nsrR) CSB-YP487549EOR
CSB-EP487549EOR
CSB-BP487549EOR
CSB-MP487549EOR
CSB-EP487549EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Iron-sulfur cluster repair protein YtfE (ytfE) CSB-YP487550EOR
CSB-EP487550EOR
CSB-BP487550EOR
CSB-MP487550EOR
CSB-EP487550EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (menH) CSB-YP487551EOR
CSB-EP487551EOR
CSB-BP487551EOR
CSB-MP487551EOR
CSB-EP487551EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Probable 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol flippase subunit ArnE (arnE), partial CSB-YP487552EOR1
CSB-EP487552EOR1
CSB-BP487552EOR1
CSB-MP487552EOR1
CSB-EP487552EOR1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Probable malate:quinone oxidoreductase (mqo), partial CSB-YP487553EOR
CSB-EP487553EOR
CSB-BP487553EOR
CSB-MP487553EOR
CSB-EP487553EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Trigger factor (tig) CSB-YP487554EOR
CSB-EP487554EOR
CSB-BP487554EOR
CSB-MP487554EOR
CSB-EP487554EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Transcriptional repressor NrdR (nrdR) CSB-YP487555EOR
CSB-EP487555EOR
CSB-BP487555EOR
CSB-MP487555EOR
CSB-EP487555EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii UPF0178 protein yaiI (yaiI) CSB-YP487556EOR
CSB-EP487556EOR
CSB-BP487556EOR
CSB-MP487556EOR
CSB-EP487556EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Arginine deiminase (arcA) CSB-YP487557EOR
CSB-EP487557EOR
CSB-BP487557EOR
CSB-MP487557EOR
CSB-EP487557EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Modulator protein MzrA (mzrA), partial CSB-YP487558EOR
CSB-EP487558EOR
CSB-BP487558EOR
CSB-MP487558EOR
CSB-EP487558EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Putative transport protein YbjL (ybjL), partial CSB-YP487559EOR
CSB-EP487559EOR
CSB-BP487559EOR
CSB-MP487559EOR
CSB-EP487559EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Glutamate 5-kinase (proB) CSB-YP487560EOR
CSB-EP487560EOR
CSB-BP487560EOR
CSB-MP487560EOR
CSB-EP487560EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Bifunctional protein aas (aas), partial CSB-YP487561EOR1
CSB-EP487561EOR1
CSB-BP487561EOR1
CSB-MP487561EOR1
CSB-EP487561EOR1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Lipoate-protein ligase A (lplA) CSB-YP487562EOR
CSB-EP487562EOR
CSB-BP487562EOR
CSB-MP487562EOR
CSB-EP487562EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Macrodomain Ter protein (matP) CSB-YP487563EOR
CSB-EP487563EOR
CSB-BP487563EOR
CSB-MP487563EOR
CSB-EP487563EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Protein phosphatase CheZ (cheZ) CSB-YP487564EOR
CSB-EP487564EOR
CSB-BP487564EOR
CSB-MP487564EOR
CSB-EP487564EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii D-erythrose-4-phosphate dehydrogenase (epd) CSB-YP487565EOR
CSB-EP487565EOR
CSB-BP487565EOR
CSB-MP487565EOR
CSB-EP487565EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Aspartate--tRNA ligase (aspS), partial CSB-YP487566EOR
CSB-EP487566EOR
CSB-BP487566EOR
CSB-MP487566EOR
CSB-EP487566EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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