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重組蛋白

產品名稱 貨號 規(guī)格
Recombinant Escherichia fergusonii 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) CSB-YP487607EOR
CSB-EP487607EOR
CSB-BP487607EOR
CSB-MP487607EOR
CSB-EP487607EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii L-alanine exporter AlaE (alaE), partial CSB-YP487608EOR1
CSB-EP487608EOR1
CSB-BP487608EOR1
CSB-MP487608EOR1
CSB-EP487608EOR1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Glutathione-regulated potassium-efflux system ancillary protein kefF (kefF) CSB-YP487609EOR
CSB-EP487609EOR
CSB-BP487609EOR
CSB-MP487609EOR
CSB-EP487609EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii NADPH-dependent 7-cyano-7-deazaguanine reductase (queF) CSB-YP487610EOR
CSB-EP487610EOR
CSB-BP487610EOR
CSB-MP487610EOR
CSB-EP487610EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Protein nrdI (nrdI) CSB-YP487611EOR
CSB-EP487611EOR
CSB-BP487611EOR
CSB-MP487611EOR
CSB-EP487611EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Pantothenate synthetase (panC) CSB-YP487612EOR
CSB-EP487612EOR
CSB-BP487612EOR
CSB-MP487612EOR
CSB-EP487612EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Outer membrane protein assembly factor yaeT (yaeT), partial CSB-YP487613EOR
CSB-EP487613EOR
CSB-BP487613EOR
CSB-MP487613EOR
CSB-EP487613EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (dapD) CSB-YP487614EOR
CSB-EP487614EOR
CSB-BP487614EOR
CSB-MP487614EOR
CSB-EP487614EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (mtnN) CSB-YP487615EOR
CSB-EP487615EOR
CSB-BP487615EOR
CSB-MP487615EOR
CSB-EP487615EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP487616EOR
CSB-EP487616EOR
CSB-BP487616EOR
CSB-MP487616EOR
CSB-EP487616EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Protein MraZ (mraZ) CSB-YP487617EOR
CSB-EP487617EOR
CSB-BP487617EOR
CSB-MP487617EOR
CSB-EP487617EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Sulfate adenylyltransferase subunit 1 (cysN) CSB-YP487618EOR
CSB-EP487618EOR
CSB-BP487618EOR
CSB-MP487618EOR
CSB-EP487618EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii 2- (5''-triphosphoribosyl)-3'-dephosphocoenzyme-A synthase CSB-YP487619EOR
CSB-EP487619EOR
CSB-BP487619EOR
CSB-MP487619EOR
CSB-EP487619EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Protein fixA (fixA) CSB-YP487620EOR
CSB-EP487620EOR
CSB-BP487620EOR
CSB-MP487620EOR
CSB-EP487620EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii GMP reductase (guaC) CSB-YP487621EOR
CSB-EP487621EOR
CSB-BP487621EOR
CSB-MP487621EOR
CSB-EP487621EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O8 Beta-hexosaminidase (nagZ) CSB-YP487622EOO
CSB-EP487622EOO
CSB-BP487622EOO
CSB-MP487622EOO
CSB-EP487622EOO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O8 Fatty acid metabolism regulator protein (fadR) CSB-YP487623EOO
CSB-EP487623EOO
CSB-BP487623EOO
CSB-MP487623EOO
CSB-EP487623EOO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O8 Outer-membrane lipoprotein lolB (lolB) CSB-YP487624EOO
CSB-EP487624EOO
CSB-BP487624EOO
CSB-MP487624EOO
CSB-EP487624EOO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O8 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD) CSB-YP487625EOO
CSB-EP487625EOO
CSB-BP487625EOO
CSB-MP487625EOO
CSB-EP487625EOO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O8 Mannonate dehydratase (uxuA) CSB-YP487626EOO
CSB-EP487626EOO
CSB-BP487626EOO
CSB-MP487626EOO
CSB-EP487626EOO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產生大量蛋白質、修改基因序列和制造有用的商業(yè)產品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產,在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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