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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Escherichia fergusonii 3-dehydroquinate synthase (aroB) CSB-YP487587EOR
CSB-EP487587EOR
CSB-BP487587EOR
CSB-MP487587EOR
CSB-EP487587EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii HTH-type transcriptional regulator malT (malT), partial CSB-YP487588EOR
CSB-EP487588EOR
CSB-BP487588EOR
CSB-MP487588EOR
CSB-EP487588EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii UPF0225 protein ychJ (ychJ) CSB-YP487589EOR
CSB-EP487589EOR
CSB-BP487589EOR
CSB-MP487589EOR
CSB-EP487589EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Cytoplasmic trehalase (treF), partial CSB-YP487590EOR
CSB-EP487590EOR
CSB-BP487590EOR
CSB-MP487590EOR
CSB-EP487590EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Flagellar P-ring protein (flgI) CSB-YP487591EOR
CSB-EP487591EOR
CSB-BP487591EOR
CSB-MP487591EOR
CSB-EP487591EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Probable phosphatase YcdX (ycdX) CSB-YP487592EOR
CSB-EP487592EOR
CSB-BP487592EOR
CSB-MP487592EOR
CSB-EP487592EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Protein-export protein secB (secB) CSB-YP487593EOR
CSB-EP487593EOR
CSB-BP487593EOR
CSB-MP487593EOR
CSB-EP487593EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Probable propionate kinase (pduW) CSB-YP487594EOR
CSB-EP487594EOR
CSB-BP487594EOR
CSB-MP487594EOR
CSB-EP487594EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Ubiquinone/menaquinone biosynthesis methyltransferase ubiE (ubiE) CSB-YP487595EOR
CSB-EP487595EOR
CSB-BP487595EOR
CSB-MP487595EOR
CSB-EP487595EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Probable UDP-N-acetyl-D-mannosaminuronic acid transferase (wecG) CSB-YP487596EOR
CSB-EP487596EOR
CSB-BP487596EOR
CSB-MP487596EOR
CSB-EP487596EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Histidinol-phosphate aminotransferase (hisC) CSB-YP487597EOR
CSB-EP487597EOR
CSB-BP487597EOR
CSB-MP487597EOR
CSB-EP487597EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Acetylornithine deacetylase (argE) CSB-YP487598EOR
CSB-EP487598EOR
CSB-BP487598EOR
CSB-MP487598EOR
CSB-EP487598EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Glycerol kinase (glpK), partial CSB-YP487599EOR
CSB-EP487599EOR
CSB-BP487599EOR
CSB-MP487599EOR
CSB-EP487599EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Autonomous glycyl radical cofactor (grcA) CSB-YP487600EOR
CSB-EP487600EOR
CSB-BP487600EOR
CSB-MP487600EOR
CSB-EP487600EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii DNA repair protein recO (recO) CSB-YP487601EOR
CSB-EP487601EOR
CSB-BP487601EOR
CSB-MP487601EOR
CSB-EP487601EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Multidrug resistance protein MdtA (mdtA) CSB-YP487602EOR
CSB-EP487602EOR
CSB-BP487602EOR
CSB-MP487602EOR
CSB-EP487602EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Methionine--tRNA ligase (metG), partial CSB-YP487603EOR
CSB-EP487603EOR
CSB-BP487603EOR
CSB-MP487603EOR
CSB-EP487603EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii L-rhamnose mutarotase (rhaM) CSB-YP487604EOR
CSB-EP487604EOR
CSB-BP487604EOR
CSB-MP487604EOR
CSB-EP487604EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Phosphopantetheine adenylyltransferase (coaD) CSB-YP487605EOR
CSB-EP487605EOR
CSB-BP487605EOR
CSB-MP487605EOR
CSB-EP487605EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii UPF0758 protein yicR (yicR) CSB-YP487606EOR
CSB-EP487606EOR
CSB-BP487606EOR
CSB-MP487606EOR
CSB-EP487606EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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