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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Brucella melitensis biotype 2 30S ribosomal protein S15 (rpsO) CSB-YP506570BWT
CSB-EP506570BWT
CSB-BP506570BWT
CSB-MP506570BWT
CSB-EP506570BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP506571BWT
CSB-EP506571BWT
CSB-BP506571BWT
CSB-MP506571BWT
CSB-EP506571BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 [Protein-PII] uridylyltransferase (glnD), partial CSB-YP506572BWT
CSB-EP506572BWT
CSB-BP506572BWT
CSB-MP506572BWT
CSB-EP506572BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 UPF0262 protein BMEA_A0286 (BMEA_A0286) CSB-YP506573BWT
CSB-EP506573BWT
CSB-BP506573BWT
CSB-MP506573BWT
CSB-EP506573BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 GTPase Der (der) CSB-YP506574BWT
CSB-EP506574BWT
CSB-BP506574BWT
CSB-MP506574BWT
CSB-EP506574BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 UPF0301 protein BMEA_A0516 (BMEA_A0516) CSB-YP506575BWT
CSB-EP506575BWT
CSB-BP506575BWT
CSB-MP506575BWT
CSB-EP506575BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Orotate phosphoribosyltransferase (pyrE) CSB-YP506576BWT
CSB-EP506576BWT
CSB-BP506576BWT
CSB-MP506576BWT
CSB-EP506576BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Aspartate--tRNA ligase (aspS), partial CSB-YP506577BWT
CSB-EP506577BWT
CSB-BP506577BWT
CSB-MP506577BWT
CSB-EP506577BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Integration host factor subunit alpha (ihfA) CSB-YP506578BWT
CSB-EP506578BWT
CSB-BP506578BWT
CSB-MP506578BWT
CSB-EP506578BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 NADH-quinone oxidoreductase subunit H (nuoH), partial CSB-YP506579BWT
CSB-EP506579BWT
CSB-BP506579BWT
CSB-MP506579BWT
CSB-EP506579BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP506580BWT
CSB-EP506580BWT
CSB-BP506580BWT
CSB-MP506580BWT
CSB-EP506580BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Serine--tRNA ligase (serS) CSB-YP506581BWT
CSB-EP506581BWT
CSB-BP506581BWT
CSB-MP506581BWT
CSB-EP506581BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Phosphoribosyl-AMP cyclohydrolase (hisI) CSB-YP506582BWT
CSB-EP506582BWT
CSB-BP506582BWT
CSB-MP506582BWT
CSB-EP506582BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Protein hfq (hfq) CSB-YP506583BWT
CSB-EP506583BWT
CSB-BP506583BWT
CSB-MP506583BWT
CSB-EP506583BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Molybdenum cofactor biosynthesis protein C (moaC) CSB-YP506584BWT
CSB-EP506584BWT
CSB-BP506584BWT
CSB-MP506584BWT
CSB-EP506584BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 30S ribosomal protein S2 (rpsB) CSB-YP506585BWT
CSB-EP506585BWT
CSB-BP506585BWT
CSB-MP506585BWT
CSB-EP506585BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Glutamate racemase (murI) CSB-YP506586BWT
CSB-EP506586BWT
CSB-BP506586BWT
CSB-MP506586BWT
CSB-EP506586BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 30S ribosomal protein S13 (rpsM) CSB-YP506587BWT
CSB-EP506587BWT
CSB-BP506587BWT
CSB-MP506587BWT
CSB-EP506587BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 30S ribosomal protein S8 (rpsH) CSB-YP506588BWT
CSB-EP506588BWT
CSB-BP506588BWT
CSB-MP506588BWT
CSB-EP506588BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 30S ribosomal protein S3 (rpsC) CSB-YP506589BWT
CSB-EP506589BWT
CSB-BP506589BWT
CSB-MP506589BWT
CSB-EP506589BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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