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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Rhodococcus erythropolis 30S ribosomal protein S14 type Z (rpsZ) CSB-YP506650RLK
CSB-EP506650RLK
CSB-BP506650RLK
CSB-MP506650RLK
CSB-EP506650RLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodococcus erythropolis 50S ribosomal protein L36 (rpmJ) CSB-YP506651RLK
CSB-EP506651RLK
CSB-BP506651RLK
CSB-MP506651RLK
CSB-EP506651RLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodococcus erythropolis 10 kDa chaperonin (groS) CSB-YP506652RLK
CSB-EP506652RLK
CSB-BP506652RLK
CSB-MP506652RLK
CSB-EP506652RLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodococcus erythropolis Peptide chain release factor 2 (prfB) CSB-YP506653RLK
CSB-EP506653RLK
CSB-BP506653RLK
CSB-MP506653RLK
CSB-EP506653RLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodococcus erythropolis Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B, partial CSB-YP506654RLK
CSB-EP506654RLK
CSB-BP506654RLK
CSB-MP506654RLK
CSB-EP506654RLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodococcus erythropolis Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP506655RLK
CSB-EP506655RLK
CSB-BP506655RLK
CSB-MP506655RLK
CSB-EP506655RLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodococcus erythropolis Ribosome-binding factor A (rbfA) CSB-YP506656RLK
CSB-EP506656RLK
CSB-BP506656RLK
CSB-MP506656RLK
CSB-EP506656RLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodococcus erythropolis Diaminopimelate epimerase (dapF) CSB-YP506657RLK
CSB-EP506657RLK
CSB-BP506657RLK
CSB-MP506657RLK
CSB-EP506657RLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodococcus erythropolis Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP506658RLK
CSB-EP506658RLK
CSB-BP506658RLK
CSB-MP506658RLK
CSB-EP506658RLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodococcus erythropolis Shikimate kinase (aroK) CSB-YP506659RLK
CSB-EP506659RLK
CSB-BP506659RLK
CSB-MP506659RLK
CSB-EP506659RLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodococcus erythropolis Riboflavin biosynthesis protein ribBA (ribBA) CSB-YP506660RLK
CSB-EP506660RLK
CSB-BP506660RLK
CSB-MP506660RLK
CSB-EP506660RLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodococcus erythropolis Prokaryotic ubiquitin-like protein Pup (pup) CSB-YP506661RLK
CSB-EP506661RLK
CSB-BP506661RLK
CSB-MP506661RLK
CSB-EP506661RLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodococcus erythropolis Probable inorganic polyphosphate/ATP-NAD kinase (ppnK) CSB-YP506662RLK
CSB-EP506662RLK
CSB-BP506662RLK
CSB-MP506662RLK
CSB-EP506662RLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodococcus erythropolis 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase CSB-YP506663RLK
CSB-EP506663RLK
CSB-BP506663RLK
CSB-MP506663RLK
CSB-EP506663RLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodococcus erythropolis L-ectoine synthase (ectC) CSB-YP506664RLK
CSB-EP506664RLK
CSB-BP506664RLK
CSB-MP506664RLK
CSB-EP506664RLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodococcus erythropolis ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP506665RLK
CSB-EP506665RLK
CSB-BP506665RLK
CSB-MP506665RLK
CSB-EP506665RLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodococcus erythropolis Arginine--tRNA ligase (argS), partial CSB-YP506666RLK
CSB-EP506666RLK
CSB-BP506666RLK
CSB-MP506666RLK
CSB-EP506666RLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodococcus erythropolis 1D-myo-inositol 2-acetamido-2-deoxy-alpha-D-glucopyranoside deacetylase (mshB) CSB-YP506667RLK
CSB-EP506667RLK
CSB-BP506667RLK
CSB-MP506667RLK
CSB-EP506667RLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gemmatimonas aurantiaca 50S ribosomal protein L35 (rpmI) CSB-YP506668GFG
CSB-EP506668GFG
CSB-BP506668GFG
CSB-MP506668GFG
CSB-EP506668GFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gemmatimonas aurantiaca 3-dehydroquinate dehydratase (aroQ) CSB-YP506669GFG
CSB-EP506669GFG
CSB-BP506669GFG
CSB-MP506669GFG
CSB-EP506669GFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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