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重組蛋白

產品名稱 貨號 規(guī)格
Recombinant Brucella melitensis biotype 2 50S ribosomal protein L10 (rplJ) CSB-YP506590BWT
CSB-EP506590BWT
CSB-BP506590BWT
CSB-MP506590BWT
CSB-EP506590BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 UPF0176 protein BMEA_B0090 (BMEA_B0090) CSB-YP506591BWT
CSB-EP506591BWT
CSB-BP506591BWT
CSB-MP506591BWT
CSB-EP506591BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 10 kDa chaperonin (groS) CSB-YP506592BWT
CSB-EP506592BWT
CSB-BP506592BWT
CSB-MP506592BWT
CSB-EP506592BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Homoserine O-succinyltransferase (metA) CSB-YP506593BWT
CSB-EP506593BWT
CSB-BP506593BWT
CSB-MP506593BWT
CSB-EP506593BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Putative Holliday junction resolvase (BMEA_B0572) CSB-YP506594BWT
CSB-EP506594BWT
CSB-BP506594BWT
CSB-MP506594BWT
CSB-EP506594BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Mannonate dehydratase (uxuA) CSB-YP506595BWT
CSB-EP506595BWT
CSB-BP506595BWT
CSB-MP506595BWT
CSB-EP506595BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Sulfoxide reductase heme-binding subunit YedZ (yedZ), partial CSB-YP506596BWT1
CSB-EP506596BWT1
CSB-BP506596BWT1
CSB-MP506596BWT1
CSB-EP506596BWT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (dapD) CSB-YP506597BWT
CSB-EP506597BWT
CSB-BP506597BWT
CSB-MP506597BWT
CSB-EP506597BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Ubiquinone/menaquinone biosynthesis methyltransferase ubiE (ubiE) CSB-YP506598BWT
CSB-EP506598BWT
CSB-BP506598BWT
CSB-MP506598BWT
CSB-EP506598BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paracoccidioides brasiliensis ATPase GET3 (GET3) CSB-YP506599ERP
CSB-EP506599ERP
CSB-BP506599ERP
CSB-MP506599ERP
CSB-EP506599ERP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paracoccidioides brasiliensis Pentafunctional AROM polypeptide (PABG_02447), partial CSB-YP506600ERP
CSB-EP506600ERP
CSB-BP506600ERP
CSB-MP506600ERP
CSB-EP506600ERP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paracoccidioides brasiliensis Mitochondrial distribution and morphology protein 34 (MDM34), partial CSB-YP506601ERP
CSB-EP506601ERP
CSB-BP506601ERP
CSB-MP506601ERP
CSB-EP506601ERP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paracoccidioides brasiliensis Probable Xaa-Pro aminopeptidase P (AMPP), partial CSB-YP506602ERP
CSB-EP506602ERP
CSB-BP506602ERP
CSB-MP506602ERP
CSB-EP506602ERP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paracoccidioides brasiliensis Pheromone-processing carboxypeptidase KEX1 (KEX1), partial CSB-YP506603ERP
CSB-EP506603ERP
CSB-BP506603ERP
CSB-MP506603ERP
CSB-EP506603ERP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paracoccidioides brasiliensis Methionine aminopeptidase 2 homolog PABG_07533 (PABG_07533) CSB-YP506604ERP
CSB-EP506604ERP
CSB-BP506604ERP
CSB-MP506604ERP
CSB-EP506604ERP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative lipoprotein yteS (yteS) CSB-YP506605BRI
CSB-EP506605BRI
CSB-BP506605BRI
CSB-MP506605BRI
CSB-EP506605BRI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative methyl-accepting chemotaxis protein yoaH (yoaH), partial CSB-YP506606BRI1
CSB-EP506606BRI1
CSB-BP506606BRI1
CSB-MP506606BRI1
CSB-EP506606BRI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Pullulanase (amyX), partial CSB-YP506607BRI
CSB-EP506607BRI
CSB-BP506607BRI
CSB-MP506607BRI
CSB-EP506607BRI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized multiple-sugar transport system permease yteP (yteP), partial CSB-YP506608BRI1
CSB-EP506608BRI1
CSB-BP506608BRI1
CSB-MP506608BRI1
CSB-EP506608BRI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana AT-rich interactive domain-containing protein 6 (ARID6) CSB-YP506609DOA
CSB-EP506609DOA
CSB-BP506609DOA
CSB-MP506609DOA
CSB-EP506609DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產生大量蛋白質、修改基因序列和制造有用的商業(yè)產品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據他們開發(fā)重組蛋白的經驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產,在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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