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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Escherichia coli N utilization substance protein B homolog (nusB) CSB-YP513313ENU
CSB-EP513313ENU
CSB-BP513313ENU
CSB-MP513313ENU
CSB-EP513313ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP513314ENU
CSB-EP513314ENU
CSB-BP513314ENU
CSB-MP513314ENU
CSB-EP513314ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Fatty acid metabolism regulator protein (fadR) CSB-YP513315ENU
CSB-EP513315ENU
CSB-BP513315ENU
CSB-MP513315ENU
CSB-EP513315ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Outer-membrane lipoprotein LolB (lolB) CSB-YP513316ENU
CSB-EP513316ENU
CSB-BP513316ENU
CSB-MP513316ENU
CSB-EP513316ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Orotidine 5'-phosphate decarboxylase (pyrF) CSB-YP513317ENU
CSB-BP513317ENU
CSB-MP513317ENU
CSB-EP513317ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Nucleoid-associated protein YejK (yejK) CSB-YP513318ENU
CSB-EP513318ENU
CSB-BP513318ENU
CSB-MP513318ENU
CSB-EP513318ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Probable malate:quinone oxidoreductase (mqo) CSB-YP513319ENU
CSB-EP513319ENU
CSB-BP513319ENU
CSB-MP513319ENU
CSB-EP513319ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Ribonuclease BN (rbn) CSB-YP513320ENU
CSB-EP513320ENU
CSB-BP513320ENU
CSB-MP513320ENU
CSB-EP513320ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Large-conductance mechanosensitive channel (mscL), partial CSB-YP513321ENU1
CSB-EP513321ENU1
CSB-BP513321ENU1
CSB-MP513321ENU1
CSB-EP513321ENU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 50S ribosomal protein L30 (rpmD) CSB-YP513322ENU
CSB-EP513322ENU
CSB-BP513322ENU
CSB-MP513322ENU
CSB-EP513322ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 30S ribosomal protein S17 (rpsQ) CSB-YP513323ENU
CSB-EP513323ENU
CSB-BP513323ENU
CSB-MP513323ENU
CSB-EP513323ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 50S ribosomal protein L4 (rplD) CSB-YP513324ENU
CSB-EP513324ENU
CSB-BP513324ENU
CSB-MP513324ENU
CSB-EP513324ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Sulfurtransferase TusD (tusD) CSB-YP513325ENU
CSB-EP513325ENU
CSB-BP513325ENU
CSB-MP513325ENU
CSB-EP513325ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Allantoinase (allB) CSB-YP513326ENU
CSB-EP513326ENU
CSB-BP513326ENU
CSB-MP513326ENU
CSB-EP513326ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UPF0207 protein yfbR (yfbR) CSB-YP513327ENU
CSB-EP513327ENU
CSB-BP513327ENU
CSB-MP513327ENU
CSB-EP513327ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli D-serine dehydratase (dsdA) CSB-YP513328ENU
CSB-EP513328ENU
CSB-BP513328ENU
CSB-MP513328ENU
CSB-EP513328ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Protein CysZ (cysZ), partial CSB-YP513329ENU1
CSB-EP513329ENU1
CSB-BP513329ENU1
CSB-MP513329ENU1
CSB-EP513329ENU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli HTH-type transcriptional regulator malT (malT), partial CSB-YP513330ENU
CSB-EP513330ENU
CSB-BP513330ENU
CSB-MP513330ENU
CSB-EP513330ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Sulfurtransferase TusA (tusA) CSB-YP513331ENU
CSB-EP513331ENU
CSB-BP513331ENU
CSB-MP513331ENU
CSB-EP513331ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Protein CrcB homolog (crcB), partial CSB-YP513332ENU1
CSB-EP513332ENU1
CSB-BP513332ENU1
CSB-MP513332ENU1
CSB-EP513332ENU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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