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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Escherichia coli Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP513333ENU
CSB-EP513333ENU
CSB-BP513333ENU
CSB-MP513333ENU
CSB-EP513333ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Potassium-transporting ATPase C chain (kdpC), partial CSB-YP513334ENU1
CSB-EP513334ENU1
CSB-BP513334ENU1
CSB-MP513334ENU1
CSB-EP513334ENU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Tautomerase pptA (pptA) CSB-YP513335ENU
CSB-EP513335ENU
CSB-BP513335ENU
CSB-MP513335ENU
CSB-EP513335ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UPF0257 lipoprotein ynfC (ynfC) CSB-YP513336ENU
CSB-EP513336ENU
CSB-BP513336ENU
CSB-MP513336ENU
CSB-EP513336ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Phosphoribosylformylglycinamidine cyclo-ligase (purM) CSB-YP513337ENU
CSB-EP513337ENU
CSB-BP513337ENU
CSB-MP513337ENU
CSB-EP513337ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) CSB-YP513338ENU
CSB-EP513338ENU
CSB-BP513338ENU
CSB-MP513338ENU
CSB-EP513338ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Co-chaperone protein hscB (hscB) CSB-YP513339ENU
CSB-EP513339ENU
CSB-BP513339ENU
CSB-MP513339ENU
CSB-EP513339ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Glyoxylate/hydroxypyruvate reductase B (ghrB) CSB-YP513340ENU
CSB-EP513340ENU
CSB-BP513340ENU
CSB-MP513340ENU
CSB-EP513340ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Phosphopantetheine adenylyltransferase (coaD) CSB-YP513341ENU
CSB-EP513341ENU
CSB-BP513341ENU
CSB-MP513341ENU
CSB-EP513341ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Purine ribonucleoside efflux pump nepI (nepI), partial CSB-YP513342ENU
CSB-EP513342ENU
CSB-BP513342ENU
CSB-MP513342ENU
CSB-EP513342ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Putative transport protein YbjL (ybjL), partial CSB-YP513343ENU
CSB-EP513343ENU
CSB-BP513343ENU
CSB-MP513343ENU
CSB-EP513343ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Hydroxylamine reductase (hcp) CSB-YP513344ENU
CSB-EP513344ENU
CSB-BP513344ENU
CSB-MP513344ENU
CSB-EP513344ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Pyridoxine/pyridoxamine 5'-phosphate oxidase (pdxH) CSB-YP513345ENU
CSB-EP513345ENU
CSB-BP513345ENU
CSB-MP513345ENU
CSB-EP513345ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Quinate/shikimate dehydrogenase (ydiB) CSB-YP513346ENU
CSB-EP513346ENU
CSB-BP513346ENU
CSB-MP513346ENU
CSB-EP513346ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP513347ENU
CSB-EP513347ENU
CSB-BP513347ENU
CSB-MP513347ENU
CSB-EP513347ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Ribosome maturation factor RimM (rimM) CSB-YP513348ENU
CSB-EP513348ENU
CSB-BP513348ENU
CSB-MP513348ENU
CSB-EP513348ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Protein nrdI (nrdI) CSB-YP513349ENU
CSB-EP513349ENU
CSB-BP513349ENU
CSB-MP513349ENU
CSB-EP513349ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Anaerobic nitric oxide reductase transcription regulator norR (norR) CSB-YP513350ENU
CSB-EP513350ENU
CSB-BP513350ENU
CSB-MP513350ENU
CSB-EP513350ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli DNA replication and repair protein recF (recF) CSB-YP513351ENU
CSB-EP513351ENU
CSB-BP513351ENU
CSB-MP513351ENU
CSB-EP513351ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Multidrug resistance protein MdtL (mdtL), partial CSB-YP513352ENU
CSB-EP513352ENU
CSB-BP513352ENU
CSB-MP513352ENU
CSB-EP513352ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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