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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Escherichia coli HTH-type transcriptional regulator hdfR (hdfR) CSB-YP513353ENU
CSB-EP513353ENU
CSB-BP513353ENU
CSB-MP513353ENU
CSB-EP513353ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Probable UDP-N-acetyl-D-mannosaminuronic acid transferase (wecG) CSB-YP513354ENU
CSB-EP513354ENU
CSB-BP513354ENU
CSB-MP513354ENU
CSB-EP513354ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli N-succinylarginine dihydrolase (astB) CSB-YP513355ENU
CSB-EP513355ENU
CSB-BP513355ENU
CSB-MP513355ENU
CSB-EP513355ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UPF0181 protein yoaH (yoaH) CSB-YP513356ENU
CSB-EP513356ENU
CSB-BP513356ENU
CSB-MP513356ENU
CSB-EP513356ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD) CSB-YP513357ENU
CSB-EP513357ENU
CSB-BP513357ENU
CSB-MP513357ENU
CSB-EP513357ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Phosphoadenosine phosphosulfate reductase (cysH) CSB-YP513358ENU
CSB-EP513358ENU
CSB-BP513358ENU
CSB-MP513358ENU
CSB-EP513358ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Protein syd (syd) CSB-YP513359ENU
CSB-EP513359ENU
CSB-BP513359ENU
CSB-MP513359ENU
CSB-EP513359ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli DNA mismatch repair protein mutH (mutH) CSB-YP513360ENU
CSB-EP513360ENU
CSB-BP513360ENU
CSB-MP513360ENU
CSB-EP513360ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Protein FdhE (fdhE) CSB-YP513361ENU
CSB-EP513361ENU
CSB-BP513361ENU
CSB-MP513361ENU
CSB-EP513361ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 2-keto-3-deoxygluconate permease (kdgT), partial CSB-YP513362ENU
CSB-EP513362ENU
CSB-BP513362ENU
CSB-MP513362ENU
CSB-EP513362ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli ATP-dependent protease subunit HslV (hslV) CSB-YP513363ENU
CSB-EP513363ENU
CSB-BP513363ENU
CSB-MP513363ENU
CSB-EP513363ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Peptide chain release factor 2 (prfB) CSB-YP513364ENU
CSB-EP513364ENU
CSB-BP513364ENU
CSB-MP513364ENU
CSB-EP513364ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Cell division protein ZapA (zapA) CSB-YP513365ENU
CSB-EP513365ENU
CSB-BP513365ENU
CSB-MP513365ENU
CSB-EP513365ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Acetylglutamate kinase (argB) CSB-YP513366ENU
CSB-EP513366ENU
CSB-BP513366ENU
CSB-MP513366ENU
CSB-EP513366ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 50S ribosomal protein L1 (rplA) CSB-YP513367ENU
CSB-EP513367ENU
CSB-BP513367ENU
CSB-MP513367ENU
CSB-EP513367ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli NADH pyrophosphatase (nudC) CSB-YP513368ENU
CSB-EP513368ENU
CSB-BP513368ENU
CSB-MP513368ENU
CSB-EP513368ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Isocitrate dehydrogenase kinase/phosphatase (aceK) CSB-YP513369ENU
CSB-EP513369ENU
CSB-BP513369ENU
CSB-MP513369ENU
CSB-EP513369ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 4-hydroxybenzoate octaprenyltransferase (ubiA), partial CSB-YP513370ENU1
CSB-EP513370ENU1
CSB-BP513370ENU1
CSB-MP513370ENU1
CSB-EP513370ENU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Fumarate reductase subunit C (frdC), partial CSB-YP513371ENU1
CSB-EP513371ENU1
CSB-BP513371ENU1
CSB-MP513371ENU1
CSB-EP513371ENU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus gammatolerans 50S ribosomal protein L37e (rpl37e) CSB-YP513372TMP
CSB-EP513372TMP
CSB-BP513372TMP
CSB-MP513372TMP
CSB-EP513372TMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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