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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacillus subtilis Uncharacterized N-acetyltransferase YesJ (yesJ) CSB-YP521719BRJ
CSB-EP521719BRJ
CSB-BP521719BRJ
CSB-MP521719BRJ
CSB-EP521719BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative ABC transporter substrate-binding protein yesO (yesO) CSB-YP521720BRJ
CSB-EP521720BRJ
CSB-BP521720BRJ
CSB-MP521720BRJ
CSB-EP521720BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Rhamnogalacturonan acetylesterase rhgT (rhgT) CSB-YP521721BRJ
CSB-EP521721BRJ
CSB-BP521721BRJ
CSB-MP521721BRJ
CSB-EP521721BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Probable rhamnogalacturonan acetylesterase yesY (yesY) CSB-YP521722BRJ
CSB-EP521722BRJ
CSB-BP521722BRJ
CSB-MP521722BRJ
CSB-EP521722BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yetH (yetH) CSB-YP521723BRJ
CSB-EP521723BRJ
CSB-BP521723BRJ
CSB-MP521723BRJ
CSB-EP521723BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized HTH-type transcriptional regulator yetL (yetL) CSB-YP521724BRJ
CSB-EP521724BRJ
CSB-BP521724BRJ
CSB-MP521724BRJ
CSB-EP521724BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Probable tRNA-dihydrouridine synthase 2 (dus2) CSB-YP521725BRJ
CSB-EP521725BRJ
CSB-BP521725BRJ
CSB-MP521725BRJ
CSB-EP521725BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis UPF0060 membrane protein YfjF (yfjF), partial CSB-YP521726BRJ1
CSB-EP521726BRJ1
CSB-BP521726BRJ1
CSB-MP521726BRJ1
CSB-EP521726BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yfjA (yfjA) CSB-YP521727BRJ
CSB-EP521727BRJ
CSB-BP521727BRJ
CSB-MP521727BRJ
CSB-EP521727BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized MFS-type transporter yfiU (yfiU), partial CSB-YP521728BRJ
CSB-EP521728BRJ
CSB-BP521728BRJ
CSB-MP521728BRJ
CSB-EP521728BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Probable siderophore transport system permease protein yfhA (yfhA), partial CSB-YP521729BRJ
CSB-EP521729BRJ
CSB-BP521729BRJ
CSB-MP521729BRJ
CSB-EP521729BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yfhH (yfhH) CSB-YP521730BRJ
CSB-EP521730BRJ
CSB-BP521730BRJ
CSB-MP521730BRJ
CSB-EP521730BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis AB hydrolase superfamily protein yfhM (yfhM) CSB-YP521731BRJ
CSB-EP521731BRJ
CSB-BP521731BRJ
CSB-MP521731BRJ
CSB-EP521731BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ygzA (ygzA) CSB-YP521732BRJ
CSB-EP521732BRJ
CSB-BP521732BRJ
CSB-MP521732BRJ
CSB-EP521732BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yhzB (yhzB) CSB-YP521733BRJ
CSB-EP521733BRJ
CSB-BP521733BRJ
CSB-MP521733BRJ
CSB-EP521733BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yhzC (yhzC) CSB-YP521734BRJ
CSB-EP521734BRJ
CSB-BP521734BRJ
CSB-MP521734BRJ
CSB-EP521734BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Regulatory protein spx (spxA) CSB-YP521735BRJ
CSB-EP521735BRJ
CSB-BP521735BRJ
CSB-MP521735BRJ
CSB-EP521735BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis UPF0413 protein yjbH (yjbH) CSB-YP521736BRJ
CSB-EP521736BRJ
CSB-BP521736BRJ
CSB-MP521736BRJ
CSB-EP521736BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis GTP pyrophosphokinase yjbM (yjbM) CSB-YP521737BRJ
CSB-EP521737BRJ
CSB-BP521737BRJ
CSB-MP521737BRJ
CSB-EP521737BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Glycine oxidase (thiO) CSB-YP521738BRJ
CSB-BP521738BRJ
CSB-MP521738BRJ
CSB-EP521738BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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