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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus subtilis N-formyl-4-amino-5-aminomethyl-2-methylpyrimidine deformylase (ylmB) CSB-YP521759BRJ
CSB-EP521759BRJ
CSB-BP521759BRJ
CSB-MP521759BRJ
CSB-EP521759BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Cell division protein SepF (sepF) CSB-YP521760BRJ
CSB-EP521760BRJ
CSB-BP521760BRJ
CSB-MP521760BRJ
CSB-EP521760BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Ribosome maturation factor RimM (rimM) CSB-YP521761BRJ
CSB-EP521761BRJ
CSB-BP521761BRJ
CSB-MP521761BRJ
CSB-EP521761BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ylqG (ylqG), partial CSB-YP521762BRJ
CSB-EP521762BRJ
CSB-BP521762BRJ
CSB-MP521762BRJ
CSB-EP521762BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Zinc metalloprotease rasP (rasP), partial CSB-YP521763BRJ1
CSB-EP521763BRJ1
CSB-BP521763BRJ1
CSB-MP521763BRJ1
CSB-EP521763BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Probable inactive metalloprotease ymfF (ymfF) CSB-YP521764BRJ
CSB-EP521764BRJ
CSB-BP521764BRJ
CSB-MP521764BRJ
CSB-EP521764BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized membrane protein ymfM (ymfM), partial CSB-YP521765BRJ1
CSB-EP521765BRJ1
CSB-BP521765BRJ1
CSB-MP521765BRJ1
CSB-EP521765BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative 8-amino-7-oxononanoate synthase/2-amino-3-ketobutyrate coenzyme A ligase (kbl) CSB-YP521766BRJ
CSB-EP521766BRJ
CSB-BP521766BRJ
CSB-MP521766BRJ
CSB-EP521766BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Polyketide synthase PksR (pksR), partial CSB-YP521767BRJ
CSB-EP521767BRJ
CSB-BP521767BRJ
CSB-MP521767BRJ
CSB-EP521767BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis UPF0714 protein ymaC (ymaC), partial CSB-YP521768BRJ
CSB-EP521768BRJ
CSB-BP521768BRJ
CSB-MP521768BRJ
CSB-EP521768BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Protein hfq (hfq) CSB-YP521769BRJ
CSB-EP521769BRJ
CSB-BP521769BRJ
CSB-MP521769BRJ
CSB-EP521769BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Probable deoxyuridine 5'-triphosphate nucleotidohydrolase yncF (yncF) CSB-YP521770BRJ
CSB-EP521770BRJ
CSB-BP521770BRJ
CSB-MP521770BRJ
CSB-EP521770BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein YndB (yndB) CSB-YP521771BRJ
CSB-EP521771BRJ
CSB-BP521771BRJ
CSB-MP521771BRJ
CSB-EP521771BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yndH (yndH) CSB-YP521772BRJ
CSB-EP521772BRJ
CSB-BP521772BRJ
CSB-MP521772BRJ
CSB-EP521772BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized membrane protein yndK (yndK), partial CSB-YP521773BRJ1
CSB-EP521773BRJ1
CSB-BP521773BRJ1
CSB-MP521773BRJ1
CSB-EP521773BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Thioredoxin-like protein yneN (yneN), partial CSB-YP521774BRJ
CSB-EP521774BRJ
CSB-BP521774BRJ
CSB-MP521774BRJ
CSB-EP521774BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative acyl-CoA synthetase YngI (yngI), partial CSB-YP521775BRJ
CSB-EP521775BRJ
CSB-BP521775BRJ
CSB-MP521775BRJ
CSB-EP521775BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis UPF0715 membrane protein yoaG (yoaG), partial CSB-YP521776BRJ1
CSB-EP521776BRJ1
CSB-BP521776BRJ1
CSB-MP521776BRJ1
CSB-EP521776BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yobB (yobB) CSB-YP521777BRJ
CSB-EP521777BRJ
CSB-BP521777BRJ
CSB-MP521777BRJ
CSB-EP521777BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yozJ (yozJ) CSB-YP521778BRJ
CSB-EP521778BRJ
CSB-BP521778BRJ
CSB-MP521778BRJ
CSB-EP521778BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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