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重組蛋白

產品名稱 貨號 規(guī)格
Recombinant 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP521699BXH
CSB-EP521699BXH
CSB-BP521699BXH
CSB-MP521699BXH
CSB-EP521699BXH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cell division protein FtsZ (ftsZ), partial CSB-YP521700BSE
CSB-EP521700BSE
CSB-BP521700BSE
CSB-MP521700BSE
CSB-EP521700BSE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum 3-carboxy-cis,cis-muconate cycloisomerase (pcaB) CSB-YP521701BVW
CSB-EP521701BVW
CSB-BP521701BVW
CSB-MP521701BVW
CSB-EP521701BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Acetoin:2,6-dichlorophenolindophenol oxidoreductase subunit alpha (acoA) CSB-YP521702BRJ
CSB-EP521702BRJ
CSB-BP521702BRJ
CSB-MP521702BRJ
CSB-EP521702BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized HTH-type transcriptional regulator yazB (yazB) CSB-YP521703BRJ
CSB-EP521703BRJ
CSB-BP521703BRJ
CSB-MP521703BRJ
CSB-EP521703BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Sporulation-killing factor biosynthesis protein skfC (skfC), partial CSB-YP521704BRJ1
CSB-EP521704BRJ1
CSB-BP521704BRJ1
CSB-MP521704BRJ1
CSB-EP521704BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ybdG (ybdG), partial CSB-YP521705BRJ1
CSB-EP521705BRJ1
CSB-BP521705BRJ1
CSB-MP521705BRJ1
CSB-EP521705BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ybdO (ybdO) CSB-YP521706BRJ
CSB-EP521706BRJ
CSB-BP521706BRJ
CSB-MP521706BRJ
CSB-EP521706BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized MFS-type transporter ybfB (ybfB), partial CSB-YP521707BRJ
CSB-EP521707BRJ
CSB-BP521707BRJ
CSB-MP521707BRJ
CSB-EP521707BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized HTH-type transcriptional regulator ybfI (ybfI) CSB-YP521708BRJ
CSB-EP521708BRJ
CSB-BP521708BRJ
CSB-MP521708BRJ
CSB-EP521708BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative hydrolase ybfO (ybfO) CSB-YP521709BRJ
CSB-EP521709BRJ
CSB-BP521709BRJ
CSB-MP521709BRJ
CSB-EP521709BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized membrane protein ybgB (ybgB), partial CSB-YP521710BRJ1
CSB-EP521710BRJ1
CSB-BP521710BRJ1
CSB-MP521710BRJ1
CSB-EP521710BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Glutaminase 1 (glsA1) CSB-YP521711BRJ
CSB-EP521711BRJ
CSB-BP521711BRJ
CSB-MP521711BRJ
CSB-EP521711BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized HTH-type transcriptional regulator ycgE (ycgE) CSB-YP521712BRJ
CSB-EP521712BRJ
CSB-BP521712BRJ
CSB-MP521712BRJ
CSB-EP521712BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yczF (yczF), partial CSB-YP521713BRJ
CSB-EP521713BRJ
CSB-BP521713BRJ
CSB-MP521713BRJ
CSB-EP521713BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative lipoprotein ydaJ (ydaJ) CSB-YP521714BRJ
CSB-EP521714BRJ
CSB-BP521714BRJ
CSB-MP521714BRJ
CSB-EP521714BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yddB (yddB), partial CSB-YP521715BRJ1
CSB-EP521715BRJ1
CSB-BP521715BRJ1
CSB-MP521715BRJ1
CSB-EP521715BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized HTH-type transcriptional regulator yezC (yezC) CSB-YP521716BRJ
CSB-EP521716BRJ
CSB-BP521716BRJ
CSB-MP521716BRJ
CSB-EP521716BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis 23S rRNA (uracil-C (5))-methyltransferase RlmCD CSB-YP521717BRJ
CSB-EP521717BRJ
CSB-BP521717BRJ
CSB-MP521717BRJ
CSB-EP521717BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yeeG (yeeG), partial CSB-YP521718BRJ
CSB-EP521718BRJ
CSB-BP521718BRJ
CSB-MP521718BRJ
CSB-EP521718BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產生大量蛋白質、修改基因序列和制造有用的商業(yè)產品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產,在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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