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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Arabidopsis thaliana Sulfite reductase [ferredoxin], chloroplastic (SIR), partial CSB-YP868080DOA
CSB-EP868080DOA
CSB-BP868080DOA
CSB-MP868080DOA
CSB-EP868080DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable methyltransferase PMT7 (At5g04060), partial CSB-YP868081DOA
CSB-EP868081DOA
CSB-BP868081DOA
CSB-MP868081DOA
CSB-EP868081DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Cell division control protein 48 homolog E (CDC48E), partial CSB-YP868082DOA
CSB-EP868082DOA
CSB-BP868082DOA
CSB-MP868082DOA
CSB-EP868082DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Xyloglucan 6-xylosyltransferase (XT1), partial CSB-YP868083DOA
CSB-EP868083DOA
CSB-BP868083DOA
CSB-MP868083DOA
CSB-EP868083DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Wall-associated receptor kinase-like 20 (WAKL20), partial CSB-YP868084DOA
CSB-EP868084DOA
CSB-BP868084DOA
CSB-MP868084DOA
CSB-EP868084DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative homeobox-leucine zipper protein ATHB-51 (ATHB-51) CSB-YP868085DOA
CSB-EP868085DOA
CSB-BP868085DOA
CSB-MP868085DOA
CSB-EP868085DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Histone H2B.7 (At3g46030) CSB-YP868086DOA
CSB-EP868086DOA
CSB-BP868086DOA
CSB-MP868086DOA
CSB-EP868086DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable WRKY transcription factor 62 (WRKY62) CSB-YP868087DOA
CSB-EP868087DOA
CSB-BP868087DOA
CSB-MP868087DOA
CSB-EP868087DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Dihydrodipicolinate synthase 1, chloroplastic (DHDPS1) CSB-YP868088DOA
CSB-EP868088DOA
CSB-BP868088DOA
CSB-MP868088DOA
CSB-EP868088DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Cation/H (+) antiporter 27 (CHX27), partial CSB-YP868089DOA
CSB-EP868089DOA
CSB-BP868089DOA
CSB-MP868089DOA
CSB-EP868089DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana LIMR family protein At5g01460 (At5g01460), partial CSB-YP868090DOA
CSB-EP868090DOA
CSB-BP868090DOA
CSB-MP868090DOA
CSB-EP868090DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Glucan endo-1,3-beta-glucosidase 7 (At4g34480) CSB-YP868091DOA
CSB-EP868091DOA
CSB-BP868091DOA
CSB-MP868091DOA
CSB-EP868091DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Wall-associated receptor kinase-like 17 (WAKL17), partial CSB-YP868092DOA
CSB-EP868092DOA
CSB-BP868092DOA
CSB-MP868092DOA
CSB-EP868092DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana RING-H2 finger protein ATL14 (ATL14), partial CSB-YP868093DOA1
CSB-EP868093DOA1
CSB-BP868093DOA1
CSB-MP868093DOA1
CSB-EP868093DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana F-box/kelch-repeat protein At4g29370 (At4g29370) CSB-YP868094DOA
CSB-EP868094DOA
CSB-BP868094DOA
CSB-MP868094DOA
CSB-EP868094DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative cysteine-rich receptor-like protein kinase 43 (CRK43), partial CSB-YP868095DOA
CSB-EP868095DOA
CSB-BP868095DOA
CSB-MP868095DOA
CSB-EP868095DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Ethylene-responsive transcription factor ERF054 (ERF054) CSB-YP868096DOA
CSB-EP868096DOA
CSB-BP868096DOA
CSB-MP868096DOA
CSB-EP868096DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Mitotic-spindle organizing protein 1B (At4g09550) CSB-YP868097DOA
CSB-EP868097DOA
CSB-BP868097DOA
CSB-MP868097DOA
CSB-EP868097DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Non-specific lipid-transfer protein 15 (LTP15) CSB-YP868098DOA
CSB-EP868098DOA
CSB-BP868098DOA
CSB-MP868098DOA
CSB-EP868098DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana DNA damage-binding protein 1a (DDB1A), partial CSB-YP868099DOA
CSB-EP868099DOA
CSB-BP868099DOA
CSB-MP868099DOA
CSB-EP868099DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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