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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Arabidopsis thaliana 40S ribosomal protein S15a-4 (RPS15AD) CSB-YP868060DOA
CSB-EP868060DOA
CSB-BP868060DOA
CSB-MP868060DOA
CSB-EP868060DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana L-type lectin-domain containing receptor kinase IX.1 (LECRK91), partial CSB-YP868061DOA
CSB-EP868061DOA
CSB-BP868061DOA
CSB-MP868061DOA
CSB-EP868061DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Transcription factor HEC3 (HEC3) CSB-YP868062DOA
CSB-EP868062DOA
CSB-BP868062DOA
CSB-MP868062DOA
CSB-EP868062DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana 40S ribosomal protein S9-1 (RPS9B) CSB-YP868063DOA
CSB-EP868063DOA
CSB-BP868063DOA
CSB-MP868063DOA
CSB-EP868063DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable CCR4-associated factor 1 homolog 9 (CAF1-9) CSB-YP868064DOA
CSB-EP868064DOA
CSB-BP868064DOA
CSB-MP868064DOA
CSB-EP868064DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Histone deacetylase 17 (HDA17) CSB-YP868065DOA
CSB-EP868065DOA
CSB-BP868065DOA
CSB-MP868065DOA
CSB-EP868065DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana F-box/LRR-repeat protein At3g58940 (At3g58940), partial CSB-YP868066DOA
CSB-EP868066DOA
CSB-BP868066DOA
CSB-MP868066DOA
CSB-EP868066DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Citrate synthase 1, peroxisomal (CSY1), partial CSB-YP868067DOA
CSB-EP868067DOA
CSB-BP868067DOA
CSB-MP868067DOA
CSB-EP868067DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Endoribonuclease Dicer homolog 3 (DCL3), partial CSB-YP868068DOA
CSB-EP868068DOA
CSB-BP868068DOA
CSB-MP868068DOA
CSB-EP868068DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana F-box protein At3g56470 (At3g56470) CSB-YP868069DOA
CSB-EP868069DOA
CSB-BP868069DOA
CSB-MP868069DOA
CSB-EP868069DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable pectinesterase 50 (PME50) CSB-YP868070DOA
CSB-EP868070DOA
CSB-BP868070DOA
CSB-MP868070DOA
CSB-EP868070DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana E3 ubiquitin-protein ligase ATL4 (ATL4), partial CSB-YP868071DOA1
CSB-EP868071DOA1
CSB-BP868071DOA1
CSB-MP868071DOA1
CSB-EP868071DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Cytokinin dehydrogenase 6 (CKX6), partial CSB-YP868072DOA
CSB-EP868072DOA
CSB-BP868072DOA
CSB-MP868072DOA
CSB-EP868072DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative peptide/nitrate transporter At5g11570 (At5g11570), partial CSB-YP868073DOA
CSB-EP868073DOA
CSB-BP868073DOA
CSB-MP868073DOA
CSB-EP868073DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Protein LURP-one-related 14 (At3g56180) CSB-YP868074DOA
CSB-EP868074DOA
CSB-BP868074DOA
CSB-MP868074DOA
CSB-EP868074DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana PRA1 family protein B6 (PRA1B6), partial CSB-YP868075DOA1
CSB-EP868075DOA1
CSB-BP868075DOA1
CSB-MP868075DOA1
CSB-EP868075DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At3g59040 (At3g59040), partial CSB-YP868076DOA
CSB-EP868076DOA
CSB-BP868076DOA
CSB-MP868076DOA
CSB-EP868076DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At5g13270, chloroplastic (PCMP-H90), partial CSB-YP868077DOA
CSB-EP868077DOA
CSB-BP868077DOA
CSB-MP868077DOA
CSB-EP868077DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana F-box/LRR-repeat protein At5g02910 (At5g02910) CSB-YP868078DOA
CSB-EP868078DOA
CSB-BP868078DOA
CSB-MP868078DOA
CSB-EP868078DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable disease resistance protein At5g04720 (At5g04720), partial CSB-YP868079DOA
CSB-EP868079DOA
CSB-BP868079DOA
CSB-MP868079DOA
CSB-EP868079DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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