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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Arabidopsis thaliana Peptide methionine sulfoxide reductase B4 (MSRB4) CSB-YP868100DOA
CSB-EP868100DOA
CSB-BP868100DOA
CSB-MP868100DOA
CSB-EP868100DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Reticulon-like protein B7 (RTNLB7), partial CSB-YP868101DOA1
CSB-EP868101DOA1
CSB-BP868101DOA1
CSB-MP868101DOA1
CSB-EP868101DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable nitrate excretion transporter 6 (At3g45710), partial CSB-YP868102DOA
CSB-EP868102DOA
CSB-BP868102DOA
CSB-MP868102DOA
CSB-EP868102DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana ABC transporter C family member 9 (ABCC9), partial CSB-YP868103DOA
CSB-EP868103DOA
CSB-BP868103DOA
CSB-MP868103DOA
CSB-EP868103DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana tRNA-splicing endonuclease subunit Sen2-1 (SEN1) CSB-YP868104DOA
CSB-EP868104DOA
CSB-BP868104DOA
CSB-MP868104DOA
CSB-EP868104DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable peptide/nitrate transporter At3g54450 (At3g54450), partial CSB-YP868105DOA
CSB-EP868105DOA
CSB-BP868105DOA
CSB-MP868105DOA
CSB-EP868105DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable boron transporter 2 (BOR2), partial CSB-YP868106DOA
CSB-EP868106DOA
CSB-BP868106DOA
CSB-MP868106DOA
CSB-EP868106DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Serpin-Z4 (At3g45220) CSB-YP868107DOA
CSB-EP868107DOA
CSB-BP868107DOA
CSB-MP868107DOA
CSB-EP868107DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Homocysteine S-methyltransferase 2 (HMT-2) CSB-YP868108DOA
CSB-EP868108DOA
CSB-BP868108DOA
CSB-MP868108DOA
CSB-EP868108DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana F-box/ankyrin repeat protein SKIP35 (SKIP35), partial CSB-YP868109DOA
CSB-EP868109DOA
CSB-BP868109DOA
CSB-MP868109DOA
CSB-EP868109DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Protein ARABIDILLO 2 (At3g60350), partial CSB-YP868110DOA
CSB-EP868110DOA
CSB-BP868110DOA
CSB-MP868110DOA
CSB-EP868110DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana ATPase 8, plasma membrane-type (AHA8), partial CSB-YP868111DOA
CSB-EP868111DOA
CSB-BP868111DOA
CSB-MP868111DOA
CSB-EP868111DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana F-box/kelch-repeat protein SKIP4 (SKIP4) CSB-YP868112DOA
CSB-EP868112DOA
CSB-BP868112DOA
CSB-MP868112DOA
CSB-EP868112DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana 40S ribosomal protein S27-2 (RPS27B) CSB-YP868113DOA
CSB-EP868113DOA
CSB-BP868113DOA
CSB-MP868113DOA
CSB-EP868113DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative calcium-transporting ATPase 11, plasma membrane-type (ACA11), partial CSB-YP868114DOA
CSB-EP868114DOA
CSB-BP868114DOA
CSB-MP868114DOA
CSB-EP868114DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable mannose-1-phosphate guanylyltransferase 2 (At3g55590) CSB-YP868115DOA
CSB-EP868115DOA
CSB-BP868115DOA
CSB-MP868115DOA
CSB-EP868115DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana CASP-like protein At3g55390 (At3g55390), partial CSB-YP868116DOA1
CSB-EP868116DOA1
CSB-BP868116DOA1
CSB-MP868116DOA1
CSB-EP868116DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Phytosulfokines 3 (PSK3) CSB-YP868117DOA
CSB-EP868117DOA
CSB-BP868117DOA
CSB-MP868117DOA
CSB-EP868117DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana F-box/LRR-repeat protein At3g48880 (At3g48880) CSB-YP868118DOA
CSB-EP868118DOA
CSB-BP868118DOA
CSB-MP868118DOA
CSB-EP868118DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Uracil phosphoribosyltransferase, chloroplastic (UPP) CSB-YP868119DOA
CSB-EP868119DOA
CSB-BP868119DOA
CSB-MP868119DOA
CSB-EP868119DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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