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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Arabidopsis thaliana Putative FBD-associated F-box protein At1g55030 (At1g55030) CSB-YP871680DOA
CSB-EP871680DOA
CSB-BP871680DOA
CSB-MP871680DOA
CSB-EP871680DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative eukaryotic translation initiation factor 4E-4 (At1g29550) CSB-YP871681DOA
CSB-EP871681DOA
CSB-BP871681DOA
CSB-MP871681DOA
CSB-EP871681DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable peptide/nitrate transporter At1g72140 (At1g72140), partial CSB-YP871682DOA
CSB-EP871682DOA
CSB-BP871682DOA
CSB-MP871682DOA
CSB-EP871682DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative F-box protein At1g33530 (At1g33530) CSB-YP871683DOA
CSB-EP871683DOA
CSB-BP871683DOA
CSB-MP871683DOA
CSB-EP871683DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana GDSL esterase/lipase At1g31550 (At1g31550) CSB-YP871684DOA
CSB-EP871684DOA
CSB-BP871684DOA
CSB-MP871684DOA
CSB-EP871684DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana PRA1 family protein F2 (PRA1F2), partial CSB-YP871685DOA1
CSB-EP871685DOA1
CSB-BP871685DOA1
CSB-MP871685DOA1
CSB-EP871685DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Glutamate receptor 3.3 (GLR3.3), partial CSB-YP871686DOA
CSB-EP871686DOA
CSB-BP871686DOA
CSB-MP871686DOA
CSB-EP871686DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana NifU-like protein 5, mitochondrial (NIFU5) CSB-YP871687DOA
CSB-EP871687DOA
CSB-BP871687DOA
CSB-MP871687DOA
CSB-EP871687DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At1g53600, mitochondrial (PCMP-E63), partial CSB-YP871688DOA
CSB-EP871688DOA
CSB-BP871688DOA
CSB-MP871688DOA
CSB-EP871688DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Auxin response factor 22 (ARF22), partial CSB-YP871689DOA
CSB-EP871689DOA
CSB-BP871689DOA
CSB-MP871689DOA
CSB-EP871689DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana LOB domain-containing protein 5 (LBD5) CSB-YP871690DOA
CSB-EP871690DOA
CSB-BP871690DOA
CSB-MP871690DOA
CSB-EP871690DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative F-box/FBD/LRR-repeat protein At1g66300 (At1g66300) CSB-YP871691DOA
CSB-EP871691DOA
CSB-BP871691DOA
CSB-MP871691DOA
CSB-EP871691DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Purple acid phosphatase 5 (PAP5) CSB-YP871692DOA
CSB-EP871692DOA
CSB-BP871692DOA
CSB-MP871692DOA
CSB-EP871692DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Cyclin-A2-4 (CYCA2-4) CSB-YP871693DOA
CSB-EP871693DOA
CSB-BP871693DOA
CSB-MP871693DOA
CSB-EP871693DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana U-box domain-containing protein 10 (PUB10), partial CSB-YP871694DOA
CSB-EP871694DOA
CSB-BP871694DOA
CSB-MP871694DOA
CSB-EP871694DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Cytosolic sulfotransferase 16 (SOT16) CSB-YP871695DOA
CSB-EP871695DOA
CSB-BP871695DOA
CSB-MP871695DOA
CSB-EP871695DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Endoglucanase 9 (CEL3) CSB-YP871696DOA
CSB-EP871696DOA
CSB-BP871696DOA
CSB-MP871696DOA
CSB-EP871696DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Cullin-3B (CUL3B), partial CSB-YP871697DOA
CSB-EP871697DOA
CSB-BP871697DOA
CSB-MP871697DOA
CSB-EP871697DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Zinc finger CCCH domain-containing protein 14 (At1g66810) CSB-YP871698DOA
CSB-EP871698DOA
CSB-BP871698DOA
CSB-MP871698DOA
CSB-EP871698DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Mitotic-spindle organizing protein 1A (At1g73790) CSB-YP871699DOA
CSB-EP871699DOA
CSB-BP871699DOA
CSB-MP871699DOA
CSB-EP871699DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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