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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Arabidopsis thaliana PHD finger protein At1g33420 (At1g33420), partial CSB-YP887235DOA
CSB-EP887235DOA
CSB-BP887235DOA
CSB-MP887235DOA
CSB-EP887235DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Protein BEARSKIN1 (BRN1) CSB-YP887236DOA
CSB-EP887236DOA
CSB-BP887236DOA
CSB-MP887236DOA
CSB-EP887236DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative pumilio homolog 17 (APUM17) CSB-YP887237DOA
CSB-EP887237DOA
CSB-BP887237DOA
CSB-MP887237DOA
CSB-EP887237DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable eukaryotic translation initiation factor 5-1 (At1g36730) CSB-YP887238DOA
CSB-EP887238DOA
CSB-BP887238DOA
CSB-MP887238DOA
CSB-EP887238DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Phospholipase A1-Igamma3, chloroplastic (At1g51440) CSB-YP887239DOA
CSB-EP887239DOA
CSB-BP887239DOA
CSB-MP887239DOA
CSB-EP887239DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana F-box/kelch-repeat protein At1g51550 (At1g51550) CSB-YP887240DOA
CSB-EP887240DOA
CSB-BP887240DOA
CSB-MP887240DOA
CSB-EP887240DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative cyclin-T1-1 (CYCT1-1) CSB-YP887241DOA
CSB-EP887241DOA
CSB-BP887241DOA
CSB-MP887241DOA
CSB-EP887241DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Alpha- (1,4)-fucosyltransferase (FUT13), partial CSB-YP887242DOA
CSB-EP887242DOA
CSB-BP887242DOA
CSB-MP887242DOA
CSB-EP887242DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Beta-glucosidase 22 (BGLU22) CSB-YP887243DOA
CSB-EP887243DOA
CSB-BP887243DOA
CSB-MP887243DOA
CSB-EP887243DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable histone H2A variant 3 (At1g52740) CSB-YP887244DOA
CSB-EP887244DOA
CSB-BP887244DOA
CSB-MP887244DOA
CSB-EP887244DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable WRKY transcription factor 57 (WRKY57) CSB-YP887245DOA
CSB-EP887245DOA
CSB-BP887245DOA
CSB-MP887245DOA
CSB-EP887245DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Actin-related protein 4A (ARP4A) CSB-YP887246DOA
CSB-EP887246DOA
CSB-BP887246DOA
CSB-MP887246DOA
CSB-EP887246DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable WRKY transcription factor 9 (WRKY9) CSB-YP887247DOA
CSB-EP887247DOA
CSB-BP887247DOA
CSB-MP887247DOA
CSB-EP887247DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Ethylene-responsive transcription factor ERF021 (ERF021) CSB-YP887248DOA
CSB-EP887248DOA
CSB-BP887248DOA
CSB-MP887248DOA
CSB-EP887248DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana E3 ubiquitin-protein ligase SINA-like 4 (At1g66650) CSB-YP887249DOA
CSB-EP887249DOA
CSB-BP887249DOA
CSB-MP887249DOA
CSB-EP887249DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana 3-oxoacyl-[acyl-carrier-protein] synthase II, chloroplastic (KAS2) CSB-YP887250DOA
CSB-EP887250DOA
CSB-BP887250DOA
CSB-MP887250DOA
CSB-EP887250DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative clathrin assembly protein At1g68110 (At1g68110) CSB-YP887251DOA
CSB-EP887251DOA
CSB-BP887251DOA
CSB-MP887251DOA
CSB-EP887251DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative zinc transporter At3g08650 (At3g08650), partial CSB-YP887252DOA
CSB-EP887252DOA
CSB-BP887252DOA
CSB-MP887252DOA
CSB-EP887252DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Glutathione S-transferase U10 (GSTU10) CSB-YP887253DOA
CSB-EP887253DOA
CSB-BP887253DOA
CSB-MP887253DOA
CSB-EP887253DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana GDSL esterase/lipase At1g74460 (At1g74460) CSB-YP887254DOA
CSB-EP887254DOA
CSB-BP887254DOA
CSB-MP887254DOA
CSB-EP887254DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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