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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant UPF0678 fatty acid-binding protein-like protein ML1006 (ML1006) CSB-YP887275MVN
CSB-EP887275MVN
CSB-BP887275MVN
CSB-MP887275MVN
CSB-EP887275MVN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Deoxyguanosinetriphosphate triphosphohydrolase-like protein (ML0831) CSB-YP887276MVN
CSB-EP887276MVN
CSB-BP887276MVN
CSB-MP887276MVN
CSB-EP887276MVN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant F420-0:gamma-glutamyl ligase (cofE) CSB-YP887277MVN
CSB-EP887277MVN
CSB-BP887277MVN
CSB-MP887277MVN
CSB-EP887277MVN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative sporulation transcription regulator WhiA (whiA) CSB-YP887278MVN
CSB-EP887278MVN
CSB-BP887278MVN
CSB-MP887278MVN
CSB-EP887278MVN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lipoprotein lprG (lprG) CSB-YP887279MVN
CSB-EP887279MVN
CSB-BP887279MVN
CSB-MP887279MVN
CSB-EP887279MVN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Carbamoyl-phosphate synthase small chain (carA) CSB-YP887280MVN
CSB-EP887280MVN
CSB-BP887280MVN
CSB-MP887280MVN
CSB-EP887280MVN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glutamine amidotransferase subunit pdxT (pdxT) CSB-YP887281MVN
CSB-EP887281MVN
CSB-BP887281MVN
CSB-MP887281MVN
CSB-EP887281MVN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein ML2537 (ML2537), partial CSB-YP887282MVN
CSB-EP887282MVN
CSB-BP887282MVN
CSB-MP887282MVN
CSB-EP887282MVN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP887283MVN
CSB-EP887283MVN
CSB-BP887283MVN
CSB-MP887283MVN
CSB-EP887283MVN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium leprae ATP-dependent DNA helicase pcrA (pcrA), partial CSB-YP887284MVN
CSB-EP887284MVN
CSB-BP887284MVN
CSB-MP887284MVN
CSB-EP887284MVN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein ML0115 (ML0115) CSB-YP887285MVN
CSB-EP887285MVN
CSB-BP887285MVN
CSB-MP887285MVN
CSB-EP887285MVN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative regulator of ribonuclease activity (ML0066) CSB-YP887286MVN
CSB-EP887286MVN
CSB-BP887286MVN
CSB-MP887286MVN
CSB-EP887286MVN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. lactis Ribonuclease HIII (rnhC) CSB-YP887287LNG
CSB-EP887287LNG
CSB-BP887287LNG
CSB-MP887287LNG
CSB-EP887287LNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. lactis Glutamine synthetase (glnA) CSB-YP887288LNG
CSB-EP887288LNG
CSB-BP887288LNG
CSB-MP887288LNG
CSB-EP887288LNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. lactis Uncharacterized RNA methyltransferase ywfF (ywfF), partial CSB-YP887289LNG
CSB-EP887289LNG
CSB-BP887289LNG
CSB-MP887289LNG
CSB-EP887289LNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. lactis Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP887290LNG
CSB-EP887290LNG
CSB-BP887290LNG
CSB-MP887290LNG
CSB-EP887290LNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. lactis Large-conductance mechanosensitive channel (mscL), partial CSB-YP887291LNG
CSB-EP887291LNG
CSB-BP887291LNG
CSB-MP887291LNG
CSB-EP887291LNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. lactis 50S ribosomal protein L29 (rpmC) CSB-YP887292LNG
CSB-EP887292LNG
CSB-BP887292LNG
CSB-MP887292LNG
CSB-EP887292LNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. lactis 50S ribosomal protein L18 (rplR) CSB-YP887293LNG
CSB-EP887293LNG
CSB-BP887293LNG
CSB-MP887293LNG
CSB-EP887293LNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. lactis UPF0177 protein yvdC (yvdC), partial CSB-YP887294LNG1
CSB-EP887294LNG1
CSB-BP887294LNG1
CSB-MP887294LNG1
CSB-EP887294LNG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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