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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Arabidopsis thaliana Probable WRKY transcription factor 26 (WRKY26) CSB-YP887215DOA
CSB-EP887215DOA
CSB-BP887215DOA
CSB-MP887215DOA
CSB-EP887215DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana DNA topoisomerase 6 subunit B (TOP6B), partial CSB-YP887216DOA
CSB-EP887216DOA
CSB-BP887216DOA
CSB-MP887216DOA
CSB-EP887216DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Auxin-responsive protein IAA34 (IAA34) CSB-YP887217DOA
CSB-EP887217DOA
CSB-BP887217DOA
CSB-MP887217DOA
CSB-EP887217DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Eukaryotic translation initiation factor 3 subunit B (TIF3B1), partial CSB-YP887218DOA
CSB-EP887218DOA
CSB-BP887218DOA
CSB-MP887218DOA
CSB-EP887218DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative F-box protein At1g46840 (At1g46840) CSB-YP887219DOA
CSB-EP887219DOA
CSB-BP887219DOA
CSB-MP887219DOA
CSB-EP887219DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana GDSL esterase/lipase At1g58430 (At1g58430) CSB-YP887220DOA
CSB-EP887220DOA
CSB-BP887220DOA
CSB-MP887220DOA
CSB-EP887220DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Transcription factor bHLH115 (BHLH115) CSB-YP887221DOA
CSB-EP887221DOA
CSB-BP887221DOA
CSB-MP887221DOA
CSB-EP887221DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana ADP-ribosylation factor GTPase-activating protein AGD2 (AGD2), partial CSB-YP887222DOA
CSB-EP887222DOA
CSB-BP887222DOA
CSB-MP887222DOA
CSB-EP887222DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana E3 ubiquitin-protein ligase SINA-like 2 (At1g66620) CSB-YP887223DOA
CSB-EP887223DOA
CSB-BP887223DOA
CSB-MP887223DOA
CSB-EP887223DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative F-box protein At1g50870 (At1g50870) CSB-YP887224DOA
CSB-EP887224DOA
CSB-BP887224DOA
CSB-MP887224DOA
CSB-EP887224DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative pentatricopeptide repeat-containing protein At1g31840 (At1g31840), partial CSB-YP887225DOA
CSB-EP887225DOA
CSB-BP887225DOA
CSB-MP887225DOA
CSB-EP887225DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana B3 domain-containing protein At1g32030 (At1g32030) CSB-YP887226DOA
CSB-EP887226DOA
CSB-BP887226DOA
CSB-MP887226DOA
CSB-EP887226DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Transcription factor bHLH83 (BHLH83) CSB-YP887227DOA
CSB-EP887227DOA
CSB-BP887227DOA
CSB-MP887227DOA
CSB-EP887227DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Protein yippee-like At3g11230 (At3g11230) CSB-YP887228DOA
CSB-EP887228DOA
CSB-BP887228DOA
CSB-MP887228DOA
CSB-EP887228DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Ankyrin repeat-containing protein At3g12360 (At3g12360), partial CSB-YP887229DOA1
CSB-EP887229DOA1
CSB-BP887229DOA1
CSB-MP887229DOA1
CSB-EP887229DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative pentatricopeptide repeat-containing protein At1g28020 (At1g28020), partial CSB-YP887230DOA
CSB-EP887230DOA
CSB-BP887230DOA
CSB-MP887230DOA
CSB-EP887230DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana MLP-like protein 168 (MLP168) CSB-YP887231DOA
CSB-EP887231DOA
CSB-BP887231DOA
CSB-MP887231DOA
CSB-EP887231DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana MACPF domain-containing protein CAD1 (CAD1), partial CSB-YP887232DOA
CSB-EP887232DOA
CSB-BP887232DOA
CSB-MP887232DOA
CSB-EP887232DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Endoplasmic oxidoreductin-1 (AERO1) CSB-YP887233DOA
CSB-EP887233DOA
CSB-BP887233DOA
CSB-MP887233DOA
CSB-EP887233DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative F-box/LRR-repeat protein At1g56400 (At1g56400) CSB-YP887234DOA
CSB-EP887234DOA
CSB-BP887234DOA
CSB-MP887234DOA
CSB-EP887234DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細(xì)胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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