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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Arabidopsis thaliana B3 domain-containing protein At5g38500 (At5g38500) CSB-YP887739DOA
CSB-EP887739DOA
CSB-BP887739DOA
CSB-MP887739DOA
CSB-EP887739DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Sucrose transport protein SUC9 (SUC9), partial CSB-YP887740DOA
CSB-EP887740DOA
CSB-BP887740DOA
CSB-MP887740DOA
CSB-EP887740DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable L-type lectin-domain containing receptor kinase S.5 (LECRKS5), partial CSB-YP887741DOA
CSB-EP887741DOA
CSB-BP887741DOA
CSB-MP887741DOA
CSB-EP887741DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Methylthioalkylmalate synthase 1, chloroplastic (MAM1) CSB-YP887742DOA
CSB-EP887742DOA
CSB-BP887742DOA
CSB-MP887742DOA
CSB-EP887742DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable disease resistance protein At5g43740 (At5g43740), partial CSB-YP887743DOA
CSB-EP887743DOA
CSB-BP887743DOA
CSB-MP887743DOA
CSB-EP887743DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Dof zinc finger protein DOF5.8 (DOF5.8) CSB-YP887744DOA
CSB-EP887744DOA
CSB-BP887744DOA
CSB-MP887744DOA
CSB-EP887744DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Nuclear transcription factor Y subunit B-2 (NFYB2) CSB-YP887745DOA
CSB-EP887745DOA
CSB-BP887745DOA
CSB-MP887745DOA
CSB-EP887745DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana ATP-dependent zinc metalloprotease FTSH 11, chloroplastic/mitochondrial (FTSH11), partial CSB-YP887746DOA1
CSB-EP887746DOA1
CSB-BP887746DOA1
CSB-MP887746DOA1
CSB-EP887746DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Bidirectional sugar transporter SWEET13 (SWEET13), partial CSB-YP887747DOA1
CSB-EP887747DOA1
CSB-BP887747DOA1
CSB-MP887747DOA1
CSB-EP887747DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative F-box protein At5g50220 (At5g50220) CSB-YP887748DOA
CSB-EP887748DOA
CSB-BP887748DOA
CSB-MP887748DOA
CSB-EP887748DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana ATP-citrate synthase beta chain protein 2 (ACLB-2), partial CSB-YP887749DOA
CSB-EP887749DOA
CSB-BP887749DOA
CSB-MP887749DOA
CSB-EP887749DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Alcohol dehydrogenase-like 7 (At5g42250) CSB-YP887750DOA
CSB-EP887750DOA
CSB-BP887750DOA
CSB-MP887750DOA
CSB-EP887750DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana WPP domain-interacting protein 2 (WIP2), partial CSB-YP887751DOA1
CSB-EP887751DOA1
CSB-BP887751DOA1
CSB-MP887751DOA1
CSB-EP887751DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana GATA transcription factor 5 (GATA5) CSB-YP887752DOA
CSB-EP887752DOA
CSB-BP887752DOA
CSB-MP887752DOA
CSB-EP887752DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative pentatricopeptide repeat-containing protein At5g65820 (At5g65820), partial CSB-YP887753DOA
CSB-EP887753DOA
CSB-BP887753DOA
CSB-MP887753DOA
CSB-EP887753DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Transcription factor HEC1 (HEC1) CSB-YP887754DOA
CSB-EP887754DOA
CSB-BP887754DOA
CSB-MP887754DOA
CSB-EP887754DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Cysteine-rich repeat secretory protein 58 (CRRSP58) CSB-YP887755DOA
CSB-EP887755DOA
CSB-BP887755DOA
CSB-MP887755DOA
CSB-EP887755DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At5g46460, mitochondrial (PCMP-H49), partial CSB-YP887756DOA
CSB-EP887756DOA
CSB-BP887756DOA
CSB-MP887756DOA
CSB-EP887756DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable disease resistance protein At5g45440 (At5g45440) CSB-YP887757DOA
CSB-EP887757DOA
CSB-BP887757DOA
CSB-MP887757DOA
CSB-EP887757DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Monocopper oxidase-like protein SKS2 (SKS2), partial CSB-YP887758DOA
CSB-EP887758DOA
CSB-BP887758DOA
CSB-MP887758DOA
CSB-EP887758DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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