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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Arabidopsis thaliana Inositol oxygenase 5 (MIOX5) CSB-YP887779DOA
CSB-EP887779DOA
CSB-BP887779DOA
CSB-MP887779DOA
CSB-EP887779DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial (FRO1) CSB-YP887780DOA
CSB-EP887780DOA
CSB-BP887780DOA
CSB-MP887780DOA
CSB-EP887780DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Protein DA1-related 6 (DAR6), partial CSB-YP887781DOA
CSB-EP887781DOA
CSB-BP887781DOA
CSB-MP887781DOA
CSB-EP887781DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Plastid division protein PDV1 (PDV1), partial CSB-YP887782DOA
CSB-EP887782DOA
CSB-BP887782DOA
CSB-MP887782DOA
CSB-EP887782DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Ras-related protein RABA1c (RABA1C) CSB-YP887783DOA
CSB-EP887783DOA
CSB-BP887783DOA
CSB-MP887783DOA
CSB-EP887783DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana E3 ubiquitin-protein ligase ORTHRUS 1 (ORTH1), partial CSB-YP887784DOA
CSB-EP887784DOA
CSB-BP887784DOA
CSB-MP887784DOA
CSB-EP887784DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At5g48730, chloroplastic (At5g48730) CSB-YP887785DOA
CSB-EP887785DOA
CSB-BP887785DOA
CSB-MP887785DOA
CSB-EP887785DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative E3 ubiquitin-protein ligase SINA-like 9 (At5g37910) CSB-YP887786DOA
CSB-EP887786DOA
CSB-BP887786DOA
CSB-MP887786DOA
CSB-EP887786DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Cyclin-U4-3 (CYCU4-3) CSB-YP887787DOA
CSB-EP887787DOA
CSB-BP887787DOA
CSB-MP887787DOA
CSB-EP887787DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana 6-phosphofructokinase 4, chloroplastic (PFK4) CSB-YP887788DOA
CSB-EP887788DOA
CSB-BP887788DOA
CSB-MP887788DOA
CSB-EP887788DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative xyloglucan endotransglucosylase/hydrolase protein 13 (XTH13) CSB-YP887789DOA
CSB-EP887789DOA
CSB-BP887789DOA
CSB-MP887789DOA
CSB-EP887789DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Cation/calcium exchanger 2 (CCX2), partial CSB-YP887790DOA
CSB-EP887790DOA
CSB-BP887790DOA
CSB-MP887790DOA
CSB-EP887790DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable S-adenosylmethionine-dependent methyltransferase At5g38780 (At5g38780) CSB-YP887791DOA
CSB-EP887791DOA
CSB-BP887791DOA
CSB-MP887791DOA
CSB-EP887791DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana KH domain-containing protein At5g56140 (At5g56140) CSB-YP887792DOA
CSB-EP887792DOA
CSB-BP887792DOA
CSB-MP887792DOA
CSB-EP887792DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Ferredoxin--NADP reductase, leaf isozyme 1, chloroplastic (LFNR1) CSB-YP887793DOA
CSB-EP887793DOA
CSB-BP887793DOA
CSB-MP887793DOA
CSB-EP887793DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Protease Do-like 9 (DEGP9), partial CSB-YP887794DOA
CSB-EP887794DOA
CSB-BP887794DOA
CSB-MP887794DOA
CSB-EP887794DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative RING-H2 finger protein ATL69 (ATL69), partial CSB-YP887795DOA1
CSB-EP887795DOA1
CSB-BP887795DOA1
CSB-MP887795DOA1
CSB-EP887795DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Defensin-like protein 69 (At5g54220) CSB-YP887796DOA
CSB-EP887796DOA
CSB-BP887796DOA
CSB-MP887796DOA
CSB-EP887796DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Expansin-A21 (EXPA21) CSB-YP887797DOA
CSB-EP887797DOA
CSB-BP887797DOA
CSB-MP887797DOA
CSB-EP887797DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative FBD-associated F-box protein At5g44940 (At5g44940) CSB-YP887798DOA
CSB-EP887798DOA
CSB-BP887798DOA
CSB-MP887798DOA
CSB-EP887798DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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