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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Arabidopsis thaliana Laccase-12 (LAC12), partial CSB-YP887799DOA
CSB-EP887799DOA
CSB-BP887799DOA
CSB-MP887799DOA
CSB-EP887799DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana U-box domain-containing protein 27 (PUB27) CSB-YP887800DOA
CSB-EP887800DOA
CSB-BP887800DOA
CSB-MP887800DOA
CSB-EP887800DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana NAC domain-containing protein 100 (NAC100) CSB-YP887801DOA
CSB-EP887801DOA
CSB-BP887801DOA
CSB-MP887801DOA
CSB-EP887801DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative purine permease 12 (PUP12), partial CSB-YP887802DOA
CSB-EP887802DOA
CSB-BP887802DOA
CSB-MP887802DOA
CSB-EP887802DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Small ubiquitin-related modifier 3 (SUMO3) CSB-YP887803DOA
CSB-EP887803DOA
CSB-BP887803DOA
CSB-MP887803DOA
CSB-EP887803DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana F-box protein At5g07610 (At5g07610) CSB-YP887804DOA
CSB-EP887804DOA
CSB-BP887804DOA
CSB-MP887804DOA
CSB-EP887804DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana DNA-binding protein BIN4 (BIN4) CSB-YP887805DOA
CSB-EP887805DOA
CSB-BP887805DOA
CSB-MP887805DOA
CSB-EP887805DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable receptor-like protein kinase At5g24010 (At5g24010), partial CSB-YP887806DOA
CSB-EP887806DOA
CSB-BP887806DOA
CSB-MP887806DOA
CSB-EP887806DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At5g52850, chloroplastic (PCMP-H31), partial CSB-YP887807DOA
CSB-EP887807DOA
CSB-BP887807DOA
CSB-MP887807DOA
CSB-EP887807DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable UDP-glucose 6-dehydrogenase 2 (UGD2) CSB-YP887808DOA
CSB-EP887808DOA
CSB-BP887808DOA
CSB-MP887808DOA
CSB-EP887808DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At5g55740, chloroplastic (CRR21), partial CSB-YP887809DOA
CSB-EP887809DOA
CSB-BP887809DOA
CSB-MP887809DOA
CSB-EP887809DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative FBD-associated F-box protein At5g56440 (At5g56440) CSB-YP887810DOA
CSB-EP887810DOA
CSB-BP887810DOA
CSB-MP887810DOA
CSB-EP887810DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Expansin-A14 (EXPA14) CSB-YP887811DOA
CSB-EP887811DOA
CSB-BP887811DOA
CSB-MP887811DOA
CSB-EP887811DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Germin-like protein subfamily 1 member 10 (At5g38930) CSB-YP887812DOA
CSB-EP887812DOA
CSB-BP887812DOA
CSB-MP887812DOA
CSB-EP887812DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Glutamine synthetase cytosolic isozyme 1-4 (GLN1-4) CSB-YP887813DOA
CSB-EP887813DOA
CSB-BP887813DOA
CSB-MP887813DOA
CSB-EP887813DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative pumilio homolog 13 (APUM13), partial CSB-YP887814DOA
CSB-EP887814DOA
CSB-BP887814DOA
CSB-MP887814DOA
CSB-EP887814DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Thioredoxin-like protein Clot (At5g42850) CSB-YP887815DOA
CSB-EP887815DOA
CSB-BP887815DOA
CSB-MP887815DOA
CSB-EP887815DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana 3-isopropylmalate dehydrogenase 3, chloroplastic (IMDH3) CSB-YP887816DOA
CSB-EP887816DOA
CSB-BP887816DOA
CSB-MP887816DOA
CSB-EP887816DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana CBS domain-containing protein CBSCBSPB1 (CBSCBSPB1), partial CSB-YP887817DOA1
CSB-EP887817DOA1
CSB-BP887817DOA1
CSB-MP887817DOA1
CSB-EP887817DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Cytochrome P450 86B1 (CYP86B1), partial CSB-YP887818DOA1
CSB-EP887818DOA1
CSB-BP887818DOA1
CSB-MP887818DOA1
CSB-EP887818DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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