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Bovine IgE ELISA Kit

  • 中文名稱:
    牛免疫球蛋白E (IgE)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-E13028B
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥4200/¥3000
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    牛免疫球蛋白E (IgE)酶聯(lián)免疫試劑盒(CSB-E13028B)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿樣本中的IgE含量。IgE即免疫球蛋白E,是介導(dǎo)I型超敏反應(yīng)的抗體。在許多過敏性疾病如哮喘、過敏性鼻炎中,IgE水平常異常升高。其研究機(jī)制主要圍繞抑制IgE生成、阻斷IgE與受體結(jié)合等方面,通過這些手段有望控制過敏癥狀、改善相關(guān)疾病病情。試劑盒檢測范圍為62.5 ng/mL-4000 ng/mL,適用于科研領(lǐng)域?qū)ε_^敏反應(yīng)機(jī)制、寄生蟲感染免疫應(yīng)答及免疫調(diào)控相關(guān)研究;研究者可通過該產(chǎn)品監(jiān)測實(shí)驗(yàn)動(dòng)物模型中IgE的動(dòng)態(tài)變化,評(píng)估免疫干預(yù)效果,或用于畜牧領(lǐng)域牛群免疫健康狀態(tài)的基礎(chǔ)研究。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫:
    IgE
  • 種屬:
    Bos taurus (Bovine)
  • 樣本類型:
    serum, plasma
  • 檢測范圍:
    62.5 ng/mL-4000 ng/mL
  • 靈敏度:
    15.6 ng/mL
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領(lǐng)域:
    Immunology
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine IgE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %95
    Range %83-102
    1:2Average %93
    Range %88-99
    1:4Average %94
    Range %90-102
    1:8Average %90
    Range %81-99
  • 回收率:
    The recovery of bovine IgE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9790-104
    EDTA plasma (n=4)9587-106
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    40002.721 2.673 2.697 2.526
    20002.169 2.042 2.106 1.935
    10001.465 1.451 1.458 1.287
    5000.928 0.906 0.917 0.746
    2500.533 0.525 0.529 0.358
    1250.390 0.378 0.384 0.213
    62.50.264 0.251 0.258 0.087
    00.173 0.168 0.171
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

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