Guinea pig Immunoglobulin E (IgE) ELISA kit

產(chǎn)品詳情

文獻(xiàn)引用 (4) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

豚鼠免疫球蛋白E(IgE)酶聯(lián)免疫試劑盒（CSB-E12102Gp）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IgE含量。試劑盒檢測范圍為93.75 ng/mL-6000 ng/mL，在豚鼠模型中常用于研究過敏性疾病機(jī)制、藥物療效評估及免疫調(diào)節(jié)實驗；科研人員可通過該產(chǎn)品探究豚鼠在過敏原暴露、免疫干預(yù)或感染性疾病過程中IgE的動態(tài)變化，為建立過敏模型、疫苗開發(fā)或免疫調(diào)控研究提供數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

IgE
種屬：

Guinea Sus scrofa (Pig)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

93.75 ng/mL-6000 ng/mL
靈敏度：

23.4 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of guinea pig IgE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	82-96
1:2	Average %	94
	Range %	91-97
1:4	Average %	103
	Range %	96-107
1:8	Average %	98
	Range %	92-102

回收率：

The recovery of guinea pig IgE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	82-96
EDTA plasma (n=4)	106	102-110

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.