Sheep Immunoglobulin E,IgE ELISA Kit

產品詳情

文獻引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

綿羊免疫球蛋白E(IgE)酶聯(lián)免疫試劑盒（CSB-E13683Sh）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IgE含量。IgE是一種在哺乳動物體內發(fā)現(xiàn)的抗體，含量極低，主要由黏膜下淋巴組織中的效應B細胞合成。它是一種親細胞抗體，可引起I型變態(tài)反應，如過敏。研究顯示，IgE與高親和力受體FcεRI相互作用是過敏反應的核心環(huán)節(jié)，其激活機制涉及FcεRI從二聚體轉變?yōu)閱误w，從而激活下游信號通路。試劑盒檢測范圍為125 ng/mL-8000 ng/mL，適用于科研領域對綿羊免疫狀態(tài)評估、過敏性疾病機制研究或寄生蟲感染模型的免疫指標分析，為畜牧學、獸醫(yī)學及比較免疫學研究提供可靠工具本品僅用于科研，不用于臨床診斷，產品具體參數(shù)及操作步驟詳見產品說明書。
縮寫：

IgE
種屬：

Ovis aries (Sheep)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

125 ng/mL-8000 ng/mL
靈敏度：

31.25 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of sheep IgE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	80-94
1:2	Average %	97
	Range %	92-100
1:4	Average %	90
	Range %	85-95
1:8	Average %	95
	Range %	87-99

回收率：

The recovery of sheep IgE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	89-102
EDTA plasma (n=4)	96	91-100

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.