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  4. Rat Immunoglobulin E,IgE ELISA Kit

Rat Immunoglobulin E,IgE ELISA Kit

  • 中文名稱(chēng)：
    大鼠免疫球蛋白E(IgE)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E07984r
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠免疫球蛋白E(IgE)酶聯(lián)免疫試劑盒（CSB-E07984r）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿樣本中的IgE含量。IgE即免疫球蛋白 E，是參與過(guò)敏性疾病發(fā)生發(fā)展的重要靶點(diǎn)。其背景在于它與速發(fā)型過(guò)敏反應(yīng)密切相關(guān)。研究機(jī)制主要是，IgE 可與肥大細(xì)胞、嗜堿性粒細(xì)胞表面受體結(jié)合，當(dāng)變應(yīng)原再次入侵，引發(fā) IgE 交聯(lián)，促使細(xì)胞釋放炎性介質(zhì)，從而導(dǎo)致過(guò)敏癥狀。試劑盒檢測(cè)范圍為3.12 ng/mL-200 ng/mL，適用于過(guò)敏機(jī)制研究、寄生蟲(chóng)感染模型評(píng)估、免疫調(diào)節(jié)藥物篩選等科研領(lǐng)域，為探索Th2型免疫反應(yīng)、肥大細(xì)胞激活等研究提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 縮寫(xiě)：
    IgE
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類(lèi)型：
    serum, plasma
  • 檢測(cè)范圍：
    3.12 ng/mL-200 ng/mL
  • 靈敏度：
    0.78 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat IgE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %85
    Range %84-93
    1:2Average %96
    Range %91-103
    1:4Average %84
    Range %81-89
    1:8Average %93
    Range %89-97
  • 回收率：
    The recovery of rat IgE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9287-98
    EDTA plasma (n=4)9590-99
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    2002.055 2.086 2.071 1.926
    1001.520 1.649 1.585 1.440
    501.101 1.156 1.129 0.984
    250.738 0.750 0.744 0.599
    12.50.417 0.445 0.431 0.286
    6.250.341 0.337 0.339 0.194
    3.120.201 0.213 0.207 0.062
    00.139 0.150 0.145 　
  • 本試劑盒所含材料：
    • A micro ELISA plate --The 96-well plate has been pre-coated with an anti-rat IgE antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
    • Two vials lyophilized standard --Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
    • One vial Biotin-labeled IgE antibody (100 x concentrate) (120 μl/bottle) --Act as the detection antibody.
    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
    • One vial Biotin-antibody Diluent (15 ml/bottle) --Dilute the high concentration Biotin-antibody to an appropriate working solution.
    • One vial HRP-avidin Diluent (15 ml/bottle) --Dilute the high concentration HRP-avidin solution to an appropriate solution.
    • One vial Sample Diluent (50 ml/bottle)--Dilute the sample to an appropriate concentration.
    • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) --- Wash away unbound or free substances.
    • One vial TMB Substrate (10 ml/bottle) --Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
    • One vial Stop Solution (10 ml/bottle) --Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells) --Cover the microplate when incubation.
    • An instruction manual

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  • 本試劑盒不含材料：
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
    • An incubator can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution

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  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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