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  4. Mouse Immunoglobulin E,IgE ELISA Kit

Mouse Immunoglobulin E,IgE ELISA Kit

  • 中文名稱：
    小鼠免疫球蛋白E(IgE)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E07983m
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠免疫球蛋白E(IgE)酶聯(lián)免疫試劑盒（CSB-E07983m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的IgE含量。IgE是介導(dǎo)Ⅰ型超敏反應(yīng)的關(guān)鍵抗體，在過敏反應(yīng)中扮演重要角色。其研究機制主要圍繞阻斷IgE與其受體結(jié)合，抑制肥大細胞和嗜堿性粒細胞活化、脫顆粒，釋放炎癥介質(zhì)，進而減輕過敏癥狀，為過敏性疾病治療提供新方向。試劑盒檢測范圍為1.56 ng/mL-100 ng/mL，該產(chǎn)品適用于科研領(lǐng)域中小鼠過敏模型構(gòu)建、免疫調(diào)節(jié)機制研究、抗過敏藥物開發(fā)等場景，特別在評估實驗動物體內(nèi)IgE水平變化、免疫應(yīng)答動態(tài)監(jiān)測等實驗中具有高特異性和穩(wěn)定性。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫：
    IgE
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma
  • 檢測范圍：
    1.56 ng/mL-100 ng/mL
  • 靈敏度：
    0.39 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse IgE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %93
    Range %89-95
    1:2Average %102
    Range %99-105
    1:4Average %93
    Range %87-96
    1:8Average %99
    Range %94-101
  • 回收率：
    The recovery of mouse IgE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9491-97
    EDTA plasma (n=4)9591-98
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    1002.435 2.328 2.382 2.245
    501.704 1.684 1.694 1.557
    251.182 1.166 1.174 1.037
    12.50.577 0.590 0.584 0.447
    6.250.400 0.410 0.405 0.268
    3.120.307 0.292 0.300 0.163
    1.560.258 0.248 0.253 0.116
    00.138 0.136 0.137 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻

產(chǎn)品評價

平均分:
0.0分 - 1 個評價

樣品類型：血清

樣品信息：小鼠

稀釋比：沒有稀釋

產(chǎn)品評價： 我用ige的elisa試劑盒檢測小鼠血清樣本，OD值梯度值很好，計算的標準曲線擬合度也很好，實驗結(jié)果濃度值與小鼠HE結(jié)果高度吻合，實驗結(jié)果可靠，不愧是國產(chǎn)大牌，下次會繼續(xù)購買。

By 李老師