Bovine complement fragment 5a (C5a) ELISA kit

產(chǎn)品詳情

文獻(xiàn)引用 (1) 說(shuō)明書(shū)下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

牛補(bǔ)體片斷5a(C5a)酶聯(lián)免疫試劑盒（CSB-EQ027637BO）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的C5a含量。C5a是補(bǔ)體系統(tǒng)激活后產(chǎn)生的重要炎性介質(zhì)。其背景在于它參與多種生理與病理過(guò)程。研究機(jī)制上，C5a能與受體結(jié)合，介導(dǎo)炎癥細(xì)胞趨化、活化，釋放炎癥因子，在感染、自身免疫病、腫瘤等疾病中發(fā)揮作用，是潛在治療靶點(diǎn)。試劑盒檢測(cè)范圍為4.69 ng/mL-300 ng/mL，該產(chǎn)品適用于動(dòng)物免疫機(jī)制研究、炎癥性疾病模型構(gòu)建、疫苗效力評(píng)估等科研領(lǐng)域，尤其為反芻動(dòng)物感染性疾病的免疫病理分析提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
縮寫(xiě)：

C5a
種屬：

Bos taurus (Bovine)
樣本類(lèi)型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

4.69 ng/mL-300 ng/mL
靈敏度：

1.17 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Immunology
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine C5a in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	88-93
1:2	Average %	101
	Range %	97-104
1:4	Average %	94
	Range %	88-99
1:8	Average %	93
	Range %	89-97

回收率：

The recovery of bovine C5a spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	92-103
EDTA plasma (n=4)	90	86-94

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.