Rabbit complement fragment 5a,C5a ELISA Kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

兔子補(bǔ)體片斷5a(C5a)酶聯(lián)免疫試劑盒（CSB-E13247Rb）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的C5a含量。C5a是補(bǔ)體系統(tǒng)激活過程中產(chǎn)生的重要炎癥介質(zhì)。其背景在于補(bǔ)體系統(tǒng)是機(jī)體免疫防御的關(guān)鍵部分。研究機(jī)制方面，C5a可與受體結(jié)合，引發(fā)炎癥細(xì)胞趨化、活化，放大炎癥反應(yīng)，在感染、自身免疫病等多種疾病病理進(jìn)程中發(fā)揮重要作用。試劑盒檢測范圍為0.625 ng/mL-40 ng/mL，科研人員可將其應(yīng)用于炎癥信號通路研究、免疫調(diào)控機(jī)制探索、疾病動(dòng)物模型評估（如感染性疾病或自身免疫模型），以及抗炎藥物研發(fā)過程中生物標(biāo)志物的動(dòng)態(tài)監(jiān)測。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

C5a
種屬：

Oryctolagus cuniculus (Rabbit)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.625 ng/mL-40 ng/mL
靈敏度：

0.156 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit C5a in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	82-90
1:2	Average %	92
	Range %	88-96
1:4	Average %	94
	Range %	90-98
1:8	Average %	102
	Range %	98-106

回收率：

The recovery of rabbit C5a spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	87	83-93
EDTA plasma (n=4)	101	97-105

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.