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  4. Rat Complement fragment 5a,C5a ELISA Kit

Rat Complement fragment 5a,C5a ELISA Kit

  • 中文名稱：
    大鼠補體片斷5a(C5a)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E08513r
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠補體片斷5a(C5a)酶聯(lián)免疫試劑盒（CSB-E08513r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的C5a含量。C5a是補體系統(tǒng)激活產(chǎn)生的重要成分。其背景在于補體系統(tǒng)是固有免疫關(guān)鍵部分。研究機制方面，C5a可與相應(yīng)受體結(jié)合，參與免疫調(diào)節(jié)、炎癥反應(yīng)等，異常激活會導(dǎo)致多種炎癥相關(guān)疾病，因此是炎癥性疾病治療的潛在靶點。試劑盒檢測范圍為1.56 ng/mL-100 ng/mL，適用于體外研究炎癥信號通路激活狀態(tài)、評估藥物對補體系統(tǒng)的干預(yù)效果，或分析疾病模型中免疫應(yīng)答動態(tài)變化，例如通過檢測實驗動物血清中C5a濃度變化揭示炎癥反應(yīng)程度，或利用細胞培養(yǎng)上清數(shù)據(jù)探究特定刺激對補體活化途徑的影響，支持科研人員在心血管疾病、神經(jīng)炎癥、免疫調(diào)節(jié)等基礎(chǔ)研究領(lǐng)域開展精準的蛋白定量分析。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    N/A
  • 縮寫：
    C5a
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍：
    1.56 ng/mL-100 ng/mL
  • 靈敏度：
    0.39 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat C5a in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1000 Average % 102  
    Range % 96-108  
    1:2000 Average % 94  
    Range % 91-98  
    1:4000 Average % 96  
    Range % 88-99  
    1:8000 Average % 92  
    Range % 86-98  
  • 回收率：
    The recovery of rat C5a spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 98 92-104  
    EDTA plasma (n=4) 94 90-98  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    100 2.241 2.140 2.191 2.053  
    50 1.804 1.703 1.754 1.616  
    25 1.215 1.204 1.210 1.072  
    12.5 0.713 0.702 0.708 0.570  
    6.25 0.464 0.475 0.470 0.332  
    3.12 0.328 0.339 0.334 0.196  
    1.56 0.245 0.251 0.248 0.110  
    0 0.137 0.138 0.138 　  
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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