Mouse Complement fragment 5a,C5a ELISA Kit
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中文名稱:小鼠補(bǔ)體片斷5a(C5a)酶聯(lián)免疫試劑盒
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貨號(hào):CSB-E08514m
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規(guī)格:96T/48T
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價(jià)格:¥3200/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:小鼠補(bǔ)體片斷5a(C5a)酶聯(lián)免疫試劑盒(CSB-E08514m)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿樣本中的C5a含量。C5a是補(bǔ)體系統(tǒng)激活過(guò)程中產(chǎn)生的重要炎癥介質(zhì)。它在免疫調(diào)節(jié)、炎癥反應(yīng)等方面發(fā)揮關(guān)鍵作用。研究機(jī)制主要聚焦于其與受體結(jié)合后引發(fā)的細(xì)胞信號(hào)傳導(dǎo)通路,如激活免疫細(xì)胞、促進(jìn)趨化等,可作為炎癥相關(guān)疾病治療的潛在靶點(diǎn)。試劑盒檢測(cè)范圍為39 pg/mL-2500 pg/mL,靈敏度為9.75 pg/mL。適用于體外實(shí)驗(yàn)中對(duì)炎癥模型、免疫調(diào)控機(jī)制或藥物干預(yù)效果的評(píng)估,為研究補(bǔ)體系統(tǒng)相關(guān)疾病的分子機(jī)制提供可靠工具本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說(shuō)明書。
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縮寫:C5a
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種屬:Mus musculus (Mouse)
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樣本類型:serum, plasma, tissue homogenates
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檢測(cè)范圍:39 pg/mL-2500 pg/mL
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靈敏度:9.75 pg/mL
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反應(yīng)時(shí)間:1-5h
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樣本體積:50-100ul
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檢測(cè)波長(zhǎng):450 nm
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研究領(lǐng)域:Immunology
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測(cè)定原理:quantitative
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測(cè)定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
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線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of mouse C5a in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:20
Average %
85
Range %
81-90
1:40
Average %
98
Range %
91-105
1:80
Average %
89
Range %
84-94
1:160
Average %
90
Range %
86-98
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回收率:
The recovery of human LIF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range
Serum (n=5)
102
97-110
EDTA plasma (n=4)
100
98-102
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標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml
OD1
OD2
Average
Corrected
2500
2.844
2.734
2.789
2.662
1250
2.331
2.197
2.264
2.137
625
1.488
1.528
1.508
1.381
312.5
0.810
0.829
0.819
0.693
156.25
0.546
0.521
0.533
0.407
78
0.357
0.363
0.360
0.234
39
0.221
0.214
0.218
0.091
0
0.128
0.125
0.127
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數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻(xiàn)
- The activation of complement C5a-C5aR1 axis in astrocytes facilitates the neuropathogenesis due to EV-A71 infection by upregulating CXCL1 P Zhu, W Ji, D Li, F Wang, T Sun, H Yang,Journal of Virology,2024
- Blockade of C5aR1 alleviates liver inflammation and fibrosis in a mouse model of NASH by regulating TLR4 signaling and macrophage polarization K Jiang,Journal of gastroenterology,2023
- Cryogel/hydrogel biomaterials and acupuncture combined to promote diabetic skin wound healing through immunomodulation TY Chen,Biomaterials,2020
- Heparin-like surface modification of polyethersulfone membrane and its biocompatibility Min Tang et al,Journal of Colloid and Interface Science,2012
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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最新研究進(jìn)展:C5a,全稱為第五組分激活肽(complement component 5a),是補(bǔ)體系統(tǒng)中的一種小分子肽類分子。當(dāng)補(bǔ)體系統(tǒng)被激活后,C5分子會(huì)被切割成C5a和C5b兩部分,C5a是其中的一個(gè)切割產(chǎn)物。C5a是一個(gè)強(qiáng)烈的炎癥介質(zhì),可以吸引和激活免疫細(xì)胞,如嗜中性粒細(xì)胞、單核細(xì)胞和巨噬細(xì)胞,促進(jìn)炎癥反應(yīng)的形成。C5a的異常高水平與許多炎癥性疾病有關(guān),例如風(fēng)濕性關(guān)節(jié)炎、肺炎、系統(tǒng)性紅斑狼瘡等。
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