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Human Complement fragment 5a,C5a ELISA Kit

  • 中文名稱:
    人補體片斷5a(C5a)酶聯(lián)免疫試劑盒
  • 貨號:
    CSB-E08512h
  • 規(guī)格:
    96T/48T
  • 價格:
    ¥3200/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    人補體片斷5a(C5a)酶聯(lián)免疫試劑盒(CSB-E08512h)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的C5a含量。C5a是補體系統(tǒng)激活后產(chǎn)生的重要活性片段。其背景在于補體系統(tǒng)是天然免疫重要組成部分。研究機制上,C5a可與相應(yīng)受體結(jié)合,在炎癥反應(yīng)、免疫調(diào)節(jié)等過程中發(fā)揮關(guān)鍵作用,參與多種疾病發(fā)生發(fā)展,是藥物研發(fā)潛在重要靶點。試劑盒檢測范圍為39 pg/ml-2500 pg/ml,靈敏度為9.75 pg/ml??蒲腥藛T可通過定量評估C5a在感染模型、自身免疫疾病研究或藥物干預(yù)實驗中的動態(tài)變化,為炎癥機制研究、免疫調(diào)節(jié)因子篩選及病理模型驗證提供數(shù)據(jù)支持本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名:
    N/A
  • 縮寫:
    C5a
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    39 pg/ml-2500 pg/ml
  • 靈敏度:
    9.75 pg/ml
  • 反應(yīng)時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領(lǐng)域:
    Immunology
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of human C5a in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

     

    Sample

    Serum(n=4)

    1:100

    Average %

    102

    Range %

    92-108

    1:200

    Average %

    94

    Range %

    89-99

    1:400

    Average %

    97

    Range %

    91-104

    1:800

    Average %

    94

    Range %

    89-98

  • 回收率:

    The recovery of human C5a spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    89

    85-92

    EDTA plasma (n=4)

    94

    90-104

  • 標(biāo)準(zhǔn)曲線:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    2500

    2.991

    3.107

    3.049

    2.920

    1250

    2.476

    2.457

    2.467

    2.338

    625

    1.423

    1.363

    1.393

    1.264

    312

    0.657

    0.643

    0.650

    0.521

    156

    0.327

    0.329

    0.328

    0.199

    78

    0.208

    0.202

    0.205

    0.076

    39

    0.171

    0.163

    0.167

    0.038

    0

    0.131

    0.127

    0.129

     

  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

引用文獻

產(chǎn)品評價

靶點詳情

  • 最新研究進展:
        C5a是補體系統(tǒng)中的一種小分子肽,是補體系統(tǒng)經(jīng)典途徑和替代途徑的共同產(chǎn)物。C5a是一種重要的炎癥介質(zhì),能夠刺激免疫細胞釋放多種促炎癥因子,如IL-1、TNF-α等,從而誘導(dǎo)炎癥反應(yīng)。此外,C5a還能夠促進免疫細胞趨化和吞噬細菌等病原體的能力。C5a的異常表達與多種炎癥性疾病的發(fā)生和發(fā)展密切相關(guān),如炎癥性腸病等。