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中文名稱:人補體片斷5a(C5a)酶聯(lián)免疫試劑盒
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貨號:CSB-E08512h
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規(guī)格:96T/48T
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價格:¥3200/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:人補體片斷5a(C5a)酶聯(lián)免疫試劑盒(CSB-E08512h)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的C5a含量。C5a是補體系統(tǒng)激活后產(chǎn)生的重要活性片段。其背景在于補體系統(tǒng)是天然免疫重要組成部分。研究機制上,C5a可與相應(yīng)受體結(jié)合,在炎癥反應(yīng)、免疫調(diào)節(jié)等過程中發(fā)揮關(guān)鍵作用,參與多種疾病發(fā)生發(fā)展,是藥物研發(fā)潛在重要靶點。試劑盒檢測范圍為39 pg/ml-2500 pg/ml,靈敏度為9.75 pg/ml??蒲腥藛T可通過定量評估C5a在感染模型、自身免疫疾病研究或藥物干預(yù)實驗中的動態(tài)變化,為炎癥機制研究、免疫調(diào)節(jié)因子篩選及病理模型驗證提供數(shù)據(jù)支持本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名:N/A
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縮寫:C5a
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種屬:Homo sapiens (Human)
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樣本類型:serum, plasma, tissue homogenates
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檢測范圍:39 pg/ml-2500 pg/ml
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靈敏度:9.75 pg/ml
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反應(yīng)時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領(lǐng)域:Immunology
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
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線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human C5a in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:100
Average %
102
Range %
92-108
1:200
Average %
94
Range %
89-99
1:400
Average %
97
Range %
91-104
1:800
Average %
94
Range %
89-98
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回收率:
The recovery of human C5a spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range
Serum (n=5)
89
85-92
EDTA plasma (n=4)
94
90-104
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標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml
OD1
OD2
Average
Corrected
2500
2.991
3.107
3.049
2.920
1250
2.476
2.457
2.467
2.338
625
1.423
1.363
1.393
1.264
312
0.657
0.643
0.650
0.521
156
0.327
0.329
0.328
0.199
78
0.208
0.202
0.205
0.076
39
0.171
0.163
0.167
0.038
0
0.131
0.127
0.129
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數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻
- Role of C5aR2 in Prognosis of Patients with Acute Respiratory Distress Syndrome through negative modulation of C5a: A Prospective Observational Study M Rashid, S Nair, PG Poojari, VS Belle, V Kunhikatta,Heliyon,2025
- The activation of complement C5a-C5aR1 axis in astrocytes facilitates the neuropathogenesis due to EV-A71 infection by upregulating CXCL1 P Zhu, W Ji, D Li, F Wang, T Sun, H Yang,Journal of Virology,2024
- Application of Hydrophilic and Low-Surface-Energy Anti-Fouling Membrane for ECMO T Sun,/,/
- Predictive value of soluble CD59 for poor 28-day neurological prognosis and all-cause mortality in patients after cardiopulmonary resuscitation: a prospective observatory study L Wang,Journal of intensive care,2023
- Higher serum PGE2 is a predicative biomarker for postoperative delirium following elective orthopedic surgery in elderly patients M Mao,BMC geriatrics,2022
- The complement C3a?C3aR and C5a?C5aR pathways promote viability and inflammation of human retinal pigment epithelium cells by targeting NF?κB signaling S Luo,Experimental and Therapeutic Medicine,2022
- Camel milk protein hydrosylate alleviates hepatic steatosis and hypertension in high fructose-fed rats MA Alshuniaber,Pharmaceutical biology,2022
- Anticoagulant Biomimetic Consecutive Gas Exchange Network for Advanced Artificial Lung Membrane Y Feng,Available at SSRN,2022
- Exploration of Molecular Mechanism of Traditional Chinese Tuina Therapy for Insomnia by Proteomics Analysis J Zhou,Research Square,2021
- Iron oxide nanoparticles improved biocompatibility and removal of middle molecule uremic toxin of polysulfone hollow fiber membranes Noresah Said, et al,Journal of Applied Polymer Science,2019
- A new approach for membrane modification based on electrochemically mediated living polymerization and self-assembly of N-tert-butyl amide-and β-cyclodextrin-involved macromolecules for blood purification FenRan, et al,Materials Science and Engineering: C,2018
- Construction and characterization of an antibacterial/anticoagulant dual-functional surface based on poly l-lactic acid electrospun fibrous mats WufengYang.et al,Materials Science and Engineering: C,2018
- Development of biocompatible and safe polyethersulfone hemodialysis membrane incorporated with functionalized multi-walled carbon nanotubes Muhammad Nidzhom Zainol Abidin.et al,Materials Science and Engineering: C,2017
- Structure and biocompatibility of poly (vinyl alcohol)-based and agarose-based monolithic composites with embedded divinylbenzene-styrene polymeric Berezhna et al,Biomaterials,2013
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靶點詳情
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最新研究進展:C5a是補體系統(tǒng)中的一種小分子肽,是補體系統(tǒng)經(jīng)典途徑和替代途徑的共同產(chǎn)物。C5a是一種重要的炎癥介質(zhì),能夠刺激免疫細胞釋放多種促炎癥因子,如IL-1、TNF-α等,從而誘導(dǎo)炎癥反應(yīng)。此外,C5a還能夠促進免疫細胞趨化和吞噬細菌等病原體的能力。C5a的異常表達與多種炎癥性疾病的發(fā)生和發(fā)展密切相關(guān),如炎癥性腸病等。
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