Pig Immunoglobulin E,IgE ELISA Kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

豬免疫球蛋白E(IgE)酶聯(lián)免疫試劑盒（CSB-E06803p）為競爭法ELISA試劑盒，定量檢測血清、血漿樣本中的IgE含量。試劑盒檢測范圍為0.049 ng/mL-12.5 ng/mL，為研究豬類過敏性疾病機(jī)制、免疫調(diào)控及疫苗效果評(píng)估提供重要依據(jù)；適用于科研場景中探究豬群免疫狀態(tài)、環(huán)境致敏原篩查、飼料過敏性評(píng)估等研究，為動(dòng)物免疫學(xué)及獸醫(yī)學(xué)研究提供高效工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

IgE
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma
檢測范圍：

0.049 ng/mL-12.5 ng/mL
靈敏度：

0.049 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Immunology
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig IgE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	92
	Range %	88-95
1:400	Average %	93
	Range %	90-97
1:800	Average %	88
	Range %	85-95
1:1600	Average %	95
	Range %	91-101

回收率：

The recovery of pig IgE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-98
EDTA plasma (n=4)	103	98-105

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.