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Chicken Corticosterone, CORT ELISA kit

  • 中文名稱：
    雞皮質(zhì)酮(CORT)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E11991C
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    雞皮質(zhì)酮/腎上腺酮(CORT)酶聯(lián)免疫試劑盒（CSB-E11991C）為競爭法ELISA試劑盒，定量檢測血清、血漿樣本中的CORT含量。試劑盒檢測范圍為0.5 ng/mL-20 ng/mL，廣泛應(yīng)用于禽類應(yīng)激生物學(xué)研究、養(yǎng)殖環(huán)境優(yōu)化評(píng)估、神經(jīng)內(nèi)分泌調(diào)控模型構(gòu)建等領(lǐng)域，為實(shí)驗(yàn)室開展家禽生理狀態(tài)監(jiān)測、行為生態(tài)學(xué)分析及激素代謝通路探索提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫：
    CORT
  • 種屬：
    Gallus gallus (Chicken)
  • 樣本類型：
    serum, plasma
  • 檢測范圍：
    0.5 ng/mL-20 ng/mL
  • 靈敏度：
    0.5 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of chicken CORT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 98  
    Range % 94-103  
    1:2 Average % 97  
    Range % 91-101  
    1:4 Average % 90  
    Range % 85-94  
    1:8 Average % 93  
    Range % 87-98  
  • 回收率：
    The recovery of chicken CORT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 84 80-88  
    EDTA plasma (n=4) 95 90-100  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    20 0.161 0.149 0.155    
    10 0.245 0.259 0.252    
    5 0.408 0.394 0.401    
    2 0.658 0.609 0.634    
    1 0.959 0.904 0.932    
    0.5 1.341 1.276 1.309    
    0 1.842 1.772 1.807    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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相關(guān)問答

■ 常見問題解答
Q:

我看到你們有用雞CORT ELISA試劑盒可以檢測EDTA血漿。那么該檢測方法是否適用肝素試管收集的血漿呢？

A:
此Elisa試劑盒可以檢測血漿，主要用EDTA抗凝劑。肝素血漿理論上可以被檢測到，最好是做梯度預(yù)實(shí)驗(yàn)驗(yàn)證一下。