Rabbit Corticosterone,CORT ELISA Kit

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產(chǎn)品詳情

產(chǎn)品描述：

兔子皮質(zhì)酮/腎上腺酮(CORT)酶聯(lián)免疫試劑盒（CSB-E06957Rb）為競爭法ELISA試劑盒，定量檢測血清、血漿樣本中的CORT含量。試劑盒檢測范圍為0.5 ng/mL-20 ng/mL，適用于神經(jīng)內(nèi)分泌研究、應(yīng)激反應(yīng)動物模型評估、藥理實驗中藥物對腎上腺軸影響的機制探討等科研場景，為探究CORT在生理病理過程中的作用提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

N/A
縮寫：

CORT
種屬：

Oryctolagus cuniculus (Rabbit)
樣本類型：

serum, plasma
檢測范圍：

0.5 ng/mL-20 ng/mL
靈敏度：

0.5 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Signal Transduction
測定原理：

quantitative
測定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit CORT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	85-105
1:2	Average %	95
	Range %	85-105
1:4	Average %	89
	Range %	83-98
1:8	Average %	90
	Range %	83-99

回收率：

The recovery of rabbit CORT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	90-110
EDTA plasma (n=4)	96	91-100