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Rat Corticosterone,CORT ELISA Kit

  • 中文名稱:
    大鼠皮質(zhì)酮(CORT)酶聯(lián)免疫試劑盒
  • 貨號:
    CSB-E07014r
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3900/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    大鼠皮質(zhì)酮/腎上腺酮(CORT)酶聯(lián)免疫試劑盒(CSB-E07014r)為競爭法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的CORT含量。CORT即皮質(zhì)酮,是一種重要的糖皮質(zhì)激素。它參與機(jī)體應(yīng)激反應(yīng)與代謝調(diào)節(jié)等生理過程。研究機(jī)制上,主要通過與細(xì)胞內(nèi)糖皮質(zhì)激素受體結(jié)合,調(diào)控相關(guān)基因表達(dá),影響細(xì)胞功能,在神經(jīng)、內(nèi)分泌等多系統(tǒng)中發(fā)揮關(guān)鍵作用。試劑盒檢測范圍為0.2 ng/mL-40 ng/mL,支持科研人員開展神經(jīng)內(nèi)分泌機(jī)制研究、慢性應(yīng)激模型評估、代謝綜合征相關(guān)實(shí)驗(yàn)及藥物干預(yù)效果驗(yàn)證等領(lǐng)域的探索,為行為學(xué)實(shí)驗(yàn)、神經(jīng)藥理學(xué)研究和激素調(diào)控機(jī)制探索提供可靠的數(shù)據(jù)支持。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名:
    N/A
  • 縮寫:
    CORT
  • 種屬:
    Rattus norvegicus (Rat)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    0.2 ng/mL-40 ng/mL
  • 靈敏度:
    0.1 ng/mL
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領(lǐng)域:
    Signal Transduction
  • 測定原理:
    quantitative
  • 測定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rat CORT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 84  
    Range % 80-91  
    1:2 Average % 90  
    Range % 85-95  
    1:4 Average % 97  
    Range % 92-103  
    1:8 Average % 105  
    Range % 100-112  
  • 回收率:
    The recovery of rat CORT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 89-99  
    EDTA plasma (n=4) 92 88-97  
                 
                 
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    40 0.138 0.132 0.135    
    10 0.239 0.258 0.249    
    3.2 0.504 0.509 0.507    
    0.8 0.939 1.009 0.974    
    0.2 1.419 1.424 1.422    
    0 2.154 2.159 2.157    
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 最新研究進(jìn)展:
    CORT是一種重要的激素,可以影響多種生理過程,如免疫和應(yīng)激反應(yīng)。最近的研究表明,CORT可以通過影響免疫細(xì)胞的活動(dòng)來調(diào)節(jié)免疫反應(yīng)。例如,在炎癥過程中,CORT可以抑制炎癥介質(zhì)的產(chǎn)生,從而緩解炎癥反應(yīng)。此外,CORT也被認(rèn)為是抑制免疫系統(tǒng)過度激活的重要因素之一。最近的研究還表明,CORT可能在多種神經(jīng)系統(tǒng)疾病中具有治療潛力,例如抑郁癥和焦慮癥等。