Pig Corticosterone,CORT ELISA Kit

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產(chǎn)品詳情

產(chǎn)品描述：

豬皮質酮/腎上腺酮(CORT)酶聯(lián)免疫試劑盒（CSB-E06812p）為競爭法ELISA試劑盒，定量檢測血清、血漿樣本中的CORT含量。CORT（皮質酮）屬糖皮質激素。在生理背景中，它參與機體應激反應等調節(jié)。研究機制方面，主要聚焦其與受體結合影響基因表達，在神經(jīng)、內分泌等多個系統(tǒng)中發(fā)揮作用，還涉及對代謝、免疫功能的調控研究。試劑盒檢測范圍為0.5 ng/mL-20 ng/mL，該產(chǎn)品可應用于科研領域，如評估養(yǎng)殖環(huán)境應激對豬生理狀態(tài)的影響、探究疾病模型中HPA軸功能變化，或用于藥理學研究中糖皮質激素代謝調控機制的探索，為動物生理學、獸醫(yī)學及比較醫(yī)學研究提供可靠的數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

N/A
縮寫：

CORT
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma
檢測范圍：

0.5 ng/mL-20 ng/mL
靈敏度：

0.5 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig CORT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	84
	Range %	80-88
1:2	Average %	92
	Range %	88-96
1:4	Average %	96
	Range %	98-100
1:8	Average %	90
	Range %	85-94

回收率：

The recovery of pig CORT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	84-93
EDTA plasma (n=4)	100	95-105

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.