Human Corticosterone,CORT ELISA Kit

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產(chǎn)品詳情

產(chǎn)品描述：

人皮質(zhì)酮/腎上腺酮(CORT)酶聯(lián)免疫試劑盒（CSB-E07968h）為競爭法ELISA試劑盒，定量檢測血清、血漿樣本中的CORT含量。CORT（皮質(zhì)酮受體）在生理上參與應(yīng)激反應(yīng)等重要活動。其研究機制主要聚焦于它在神經(jīng)內(nèi)分泌系統(tǒng)中的信號傳導(dǎo)途徑，探究其與糖皮質(zhì)激素的結(jié)合作用，以及對下游基因表達調(diào)控的影響，有助于理解應(yīng)激相關(guān)疾病的發(fā)病機理。試劑盒檢測范圍為0.5 ng/mL-20 ng/mL，適用于神經(jīng)內(nèi)分泌研究、代謝綜合征模型構(gòu)建、慢性壓力相關(guān)動物實驗等科研場景，為探究HPA軸（下丘腦 - 垂體 - 腎上腺軸）功能、糖皮質(zhì)激素代謝通路及藥物干預(yù)效果提供高效工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

N/A
縮寫：

CORT
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma
檢測范圍：

0.5 ng/mL-20 ng/mL
靈敏度：

0.5 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Signal Transduction
測定原理：

quantitative
測定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human CORT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	103
	Range %	99-108
1:2	Average %	92
	Range %	88-96
1:4	Average %	96
	Range %	98-100
1:8	Average %	90
	Range %	85-94

回收率：

The recovery of human CORT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	84-93
EDTA plasma (n=4)	94	90-100

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.