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  2. 試劑盒
  3. 酶聯(lián)免疫試劑盒
  4. Mouse Corticosterone,CORT ELISA Kit
  • 中文名稱：
    小鼠皮質(zhì)酮(CORT)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E07969m
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠皮質(zhì)酮/腎上腺酮(CORT)酶聯(lián)免疫試劑盒（CSB-E07969m）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的CORT含量。試劑盒檢測(cè)范圍為15.6 ng/ml-1000 ng/ml，適用于體外分析多種生物樣本中的CORT水平，尤其適用于評(píng)估應(yīng)激反應(yīng)模型、慢性代謝疾病研究或藥物干預(yù)實(shí)驗(yàn)中的腎上腺功能狀態(tài)，可滿足實(shí)驗(yàn)室對(duì)小鼠模型應(yīng)激水平、激素代謝動(dòng)態(tài)及病理生理機(jī)制研究的精準(zhǔn)檢測(cè)需求本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    N/A
  • 縮寫(xiě)：
    CORT
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    15.6 ng/ml-1000 ng/ml.
  • 靈敏度：
    3.9 ng/ml.
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Competitive
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse CORT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1

    Average %

    84

    Range %

    80-92

    1:2

    Average %

    87

    Range %

    81-95

    1:4

    Average %

    85

    Range %

    82-93

    1:8

    Average %

    88

    Range %

    82-98

  • 回收率：

    The recovery of mouse CORT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    90

    86-98

    EDTA plasma (n=4)

    88

    83-96

  • 標(biāo)準(zhǔn)曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    ng/ml

    OD1

    OD2

    Average

    1000

    0.150

    0.144

    0.147

    500

    0.185

    0.182

    0.183

    250

    0.261

    0.248

    0.254

    125

    0.389

    0.400

    0.395

    62.5

    0.642

    0.621

    0.632

    31.25

    1.004

    1.053

    1.028

    15.6

    1.410

    1.384

    1.397

    0

    2.166

    2.125

    2.145

  • 本試劑盒所含材料：
      • A micro ELISA plate --- The 96-well plate has been pre-coated with goat anti-rabbit CORT antibody.
      • Six vials lyophilized standard (0.5 ml/bottle) --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
      • Anti-mouse CORT Antibody (1 x 6 ml) --- Act as the detection antibody.
      • HRP-conjugate (1 x 6 ml) --- HRP catalyzes TMB substrate to elicit a chromogenic reaction.
      • Wash Buffer (20 x concentrate) (1 x 15 ml) --- Wash away unbound or free substances.
      • Substrate A (1 x 7 ml) --- Mix with substrate B, and the TMB is catalyzed by HRP to elicit a chromogenic signal.
      • Substrate B (1 x 7 ml) --- Mix with substrate A, and the TMB is catalyzed by HRP to elicit a chromogenic signal.
      • Stop Solution 1 x 7 ml --- Stop the color development, and the eventual solution color turns yellow.
      • Four Adhesive Strips (For 96 wells) --- Cover the microwell plate when incubation.
      • An Instruction manual

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  • 本試劑盒不含材料：
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm to 630 nm.
      • An incubator that can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評(píng)價(jià)

平均分:
5.0分 - 1 個(gè)評(píng)價(jià)

樣品類型：血清

樣品信息：小鼠

稀釋比：其他 1:25

產(chǎn)品評(píng)價(jià)： 使用CSB-E07969m試劑盒檢測(cè)小鼠血清CORT水平，稀釋25倍后大部分均在標(biāo)曲范圍內(nèi)，但如果樣品充足可考慮1:10稀釋。全部實(shí)驗(yàn)在3.5小時(shí)內(nèi)完成，檢測(cè)結(jié)果較穩(wěn)定，標(biāo)曲良好.

By 蘭老師

靶點(diǎn)詳情

  • 最新研究進(jìn)展：
    CORT是一種主要由腎上腺皮質(zhì)合成的類固醇激素，具有重要的生理功能，例如調(diào)節(jié)葡萄糖代謝、免疫系統(tǒng)和心血管系統(tǒng)等。最新研究表明，CORT在多種疾病的發(fā)生和發(fā)展中也發(fā)揮著重要作用。例如，CORT與焦慮和抑郁等心理疾病的發(fā)病機(jī)制有關(guān)。此外，一些研究還發(fā)現(xiàn)CORT在自身免疫性疾病、腫瘤、神經(jīng)系統(tǒng)疾病和心血管疾病等方面發(fā)揮著重要作用。